Magnesium in PDB 8bze: Fc-J Stabilizer For Era and 14-3-3

The structure of Fc-J Stabilizer For Era and 14-3-3, PDB code: 8bze was solved by E.J.Visser, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.40 / 1.43
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.971, 111.992, 62.431, 90, 90, 90
R / Rfree (%)	17.5 / 19

The binding sites of Magnesium atom in the Fc-J Stabilizer For Era and 14-3-3 (pdb code 8bze). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Fc-J Stabilizer For Era and 14-3-3, PDB code: 8bze:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Fc-J Stabilizer For Era and 14-3-3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Fc-J Stabilizer For Era and 14-3-3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:32.9 occ:1.00 O A:HOH448 3.2 16.8 1.0 O A:HOH536 3.4 33.4 1.0 O A:HOH659 3.5 33.9 1.0 HZ3 A:LYS159 3.8 49.6 1.0 HZ1 A:LYS159 4.3 49.6 1.0 NZ A:LYS159 4.4 41.4 1.0 O A:HOH727 4.4 28.1 1.0 O A:HOH646 4.5 28.7 1.0 HZ2 A:LYS159 4.6 49.6 1.0 O A:HOH596 4.7 28.9 1.0 O A:HOH613 4.7 32.5 1.0 OE2 A:GLU189 4.8 17.5 1.0 O A:HOH676 4.9 40.1 1.0

Magnesium binding site 2 out of 3 in the Fc-J Stabilizer For Era and 14-3-3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Fc-J Stabilizer For Era and 14-3-3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:17.8 occ:0.74 O A:HOH569 2.3 25.4 1.0 OE1 A:GLU2 2.6 16.8 0.5 OE1 A:GLU2 2.6 16.8 0.5 O A:HOH427 2.7 18.5 1.0 CD A:GLU2 3.5 18.6 0.5 CD A:GLU2 3.5 18.6 0.5 OE2 A:GLU2 3.9 18.2 0.5 OE2 A:GLU2 3.9 18.2 0.5 HA A:GLU2 4.0 15.2 0.5 HA A:GLU2 4.0 15.2 0.5 H A:ARG3 4.1 16.7 1.0 O A:HOH593 4.5 20.0 1.0 O A:HOH716 4.6 19.8 1.0 O A:HOH662 4.7 35.0 1.0 CG A:GLU2 4.8 14.5 0.5 CG A:GLU2 4.8 14.5 0.5 HB3 A:GLU2 4.8 17.7 0.5 HB3 A:GLU2 4.8 17.7 0.5 CA A:GLU2 4.9 12.6 0.5 CA A:GLU2 4.9 12.6 0.5 N A:ARG3 4.9 13.9 1.0 HG2 A:GLU2 5.0 17.4 0.5 HG2 A:GLU2 5.0 17.4 0.5

Magnesium binding site 3 out of 3 in the Fc-J Stabilizer For Era and 14-3-3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Fc-J Stabilizer For Era and 14-3-3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:23.4 occ:1.00 O A:HOH440 2.2 27.4 1.0 O A:HOH658 2.3 31.3 1.0 OE2 A:GLU89 2.3 20.6 1.0 O A:HOH631 2.4 31.2 1.0 CD A:GLU89 3.4 14.7 1.0 HG2 A:GLU86 3.6 22.0 1.0 HG2 A:GLU89 3.7 19.1 1.0 HH12 A:ARG85 3.9 24.4 1.0 O A:HOH519 4.0 34.8 1.0 HE22 A:GLN93 4.1 22.0 1.0 CG A:GLU89 4.1 15.9 1.0 OE1 A:GLU89 4.3 16.0 1.0 HH11 A:ARG85 4.3 24.4 1.0 HB3 A:GLU89 4.3 18.7 1.0 HA A:GLU86 4.4 18.0 1.0 NH1 A:ARG85 4.5 20.3 1.0 OE1 A:GLN93 4.5 18.4 1.0 HG1 A:THR90 4.5 24.7 1.0 O A:HOH636 4.5 30.8 1.0 O A:HOH619 4.5 33.0 1.0 CG A:GLU86 4.6 18.3 1.0 NE2 A:GLN93 4.7 18.3 1.0 HB3 A:GLU86 4.8 20.2 1.0 OG1 A:THR90 4.8 20.6 1.0 CB A:GLU89 4.8 15.6 1.0 HG3 A:GLU89 4.9 19.1 1.0 CD A:GLN93 5.0 17.8 1.0

E.J.Visser, M.Donaldson Collier, J.Siefert, M.Konstantinidou, S.Paul, P.Cossar, G.Miley, O.Meijer, M.R.Arkin, C.Ottmann, W.Zwart, L.Brunsveld. Stabilization of the Estrogen Receptor Alpha - 14-3-3 Interaction As A New Potential Intervention Strategy For Endocrine Resistance in Breast Cancer To Be Published.