Magnesium in PDB 8bzt: Fc-Nag Stabilizer of 14-3-3 and Eralpha

The structure of Fc-Nag Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzt was solved by E.J.Visser, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.33 / 1.65
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.561, 111.572, 62.495, 90, 90, 90
R / Rfree (%)	18.3 / 20.8

The binding sites of Magnesium atom in the Fc-Nag Stabilizer of 14-3-3 and Eralpha (pdb code 8bzt). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Fc-Nag Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzt:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Fc-Nag Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Fc-Nag Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:37.8 occ:1.00 O A:HOH442 3.1 20.7 1.0 O A:HOH626 3.3 40.1 1.0 O A:HOH518 3.5 41.1 1.0 HZ1 A:LYS159 3.8 48.8 1.0 HZ3 A:LYS159 4.3 48.8 1.0 O A:HOH657 4.3 33.1 1.0 NZ A:LYS159 4.3 40.7 1.0 O A:HOH455 4.4 34.8 1.0 HZ2 A:LYS159 4.4 48.8 1.0 O A:HOH606 4.5 31.5 1.0 O A:HOH602 4.6 44.4 1.0 OE2 A:GLU189 4.7 22.4 1.0

Magnesium binding site 2 out of 3 in the Fc-Nag Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Fc-Nag Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:24.7 occ:0.71 O A:HOH541 2.3 37.4 1.0 OE1 A:GLU2 2.4 23.4 1.0 O A:HOH431 2.5 23.5 1.0 CD A:GLU2 3.4 25.6 1.0 OE2 A:GLU2 3.8 23.8 1.0 HA A:GLU2 4.0 21.2 1.0 H A:ARG3 4.2 21.6 1.0 O A:HOH564 4.4 24.9 1.0 O A:HOH438 4.4 42.8 1.0 O A:HOH511 4.4 25.1 1.0 CG A:GLU2 4.6 18.6 1.0 HB3 A:GLU2 4.7 23.7 1.0 HG2 A:GLU2 4.8 22.3 1.0 CA A:GLU2 4.9 17.7 1.0 N A:ARG3 5.0 18.0 1.0 CB A:GLU2 5.0 19.7 1.0

Magnesium binding site 3 out of 3 in the Fc-Nag Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Fc-Nag Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:28.5 occ:1.00 O A:HOH484 2.1 36.5 1.0 O A:HOH622 2.2 36.3 1.0 O A:HOH590 2.4 40.4 1.0 OE2 A:GLU89 2.4 25.1 1.0 CD A:GLU89 3.4 23.5 1.0 HG2 A:GLU86 3.6 26.9 1.0 HG2 A:GLU89 3.8 25.8 1.0 HH12 A:ARG85 3.9 30.1 1.0 HE22 A:GLN93 3.9 31.1 1.0 O A:HOH589 4.1 44.0 1.0 CG A:GLU89 4.2 21.5 1.0 HB3 A:GLU89 4.2 23.9 1.0 HH11 A:ARG85 4.3 30.1 1.0 OE1 A:GLU89 4.3 21.5 1.0 O A:HOH598 4.3 37.7 1.0 HA A:GLU86 4.4 21.9 1.0 NH1 A:ARG85 4.5 25.1 1.0 HG1 A:THR90 4.5 30.5 1.0 OE1 A:GLN93 4.5 21.4 1.0 O A:HOH588 4.6 35.7 1.0 CG A:GLU86 4.6 22.4 1.0 NE2 A:GLN93 4.6 25.9 1.0 OG1 A:THR90 4.8 25.4 1.0 CB A:GLU89 4.8 19.9 1.0 HB3 A:GLU86 4.8 24.6 1.0 O A:HOH410 4.9 40.4 1.0 CD A:GLN93 5.0 23.4 1.0 HG3 A:GLU89 5.0 25.8 1.0

E.J.Visser, M.Donaldson Collier, J.Siefert, M.Konstantinidou, S.Paul, P.Cossar, G.Miley, O.Meijer, M.R.Arkin, C.Ottmann, W.Zwart, L.Brunsveld. Stabilization of the Estrogen Receptor Alpha - 14-3-3 Interaction As A Potential Intervention Strategy For Endocrine Resistance in Breast Cancer To Be Published.