Magnesium in PDB 8cp9: 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp.

All present enzymatic activity of 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp.:
2.7.1.170;

The structure of 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp., PDB code: 8cp9 was solved by E.Jimenez-Faraco, J.A.Hermoso, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.02 / 2.20
Space group	P 63
Cell size a, b, c (Å), α, β, γ (°)	89.94, 89.94, 176.958, 90, 90, 120
R / Rfree (%)	18.3 / 23.1

The binding sites of Magnesium atom in the 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp. (pdb code 8cp9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp., PDB code: 8cp9:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:60.4 occ:1.00 O2A A:ANP401 2.1 53.3 1.0 O A:HOH504 2.1 36.9 1.0 O A:HOH515 2.1 51.9 1.0 OD1 A:ASP14 2.4 49.3 1.0 O2B A:ANP401 2.5 70.7 1.0 O A:HOH506 2.5 47.1 1.0 O1B A:ANP401 2.6 60.4 1.0 PB A:ANP401 2.8 52.2 1.0 PA A:ANP401 3.0 48.4 1.0 O3A A:ANP401 3.1 58.5 1.0 CG A:ASP14 3.5 49.1 1.0 O A:HOH542 3.8 23.6 1.0 OD2 A:ASP14 3.9 45.5 1.0 OD2 A:ASP17 4.0 48.7 1.0 N A:GLY10 4.0 42.1 1.0 O1A A:ANP401 4.1 54.6 1.0 O5' A:ANP401 4.2 53.5 1.0 CA A:GLY10 4.3 43.0 1.0 C5' A:ANP401 4.4 53.4 1.0 N3B A:ANP401 4.4 63.1 1.0 O A:GLY15 4.5 35.0 1.0 CB A:ASP14 4.7 45.0 1.0 CB A:SER12 4.8 35.7 1.0 OE1 A:GLU326 4.8 44.5 1.0 C A:GLY10 4.8 43.9 1.0 CG A:ASP17 4.8 48.0 1.0 O A:GLY10 4.9 46.2 1.0 N A:ASP14 4.9 42.6 1.0

Magnesium binding site 2 out of 2 in the 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 1,6-Anhydro-N-Actetylmuramic Acid Kinase (Anmk)in Complex with Non- Hydrolyzable Amppnp. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:57.8 occ:1.00 O B:HOH518 1.9 48.8 1.0 O B:HOH537 2.1 43.1 1.0 O2A B:ANP401 2.1 47.6 1.0 O1B B:ANP401 2.2 51.3 1.0 OD1 B:ASP14 2.4 51.1 1.0 CG B:ASP14 3.2 47.9 1.0 OD2 B:ASP14 3.3 51.7 1.0 PA B:ANP401 3.4 43.4 1.0 PB B:ANP401 3.4 47.1 1.0 O3A B:ANP401 3.7 42.2 1.0 N B:GLY10 4.0 37.4 1.0 OD2 B:ASP17 4.0 56.1 1.0 N3B B:ANP401 4.1 54.4 1.0 O B:GLY15 4.2 39.4 1.0 CA B:GLY10 4.4 37.1 1.0 O5' B:ANP401 4.4 46.8 1.0 C5' B:ANP401 4.4 47.7 1.0 O1A B:ANP401 4.4 47.9 1.0 OD1 B:ASP17 4.5 47.9 1.0 CB B:ASP14 4.6 48.3 1.0 O2B B:ANP401 4.7 46.0 1.0 CG B:ASP17 4.7 49.3 1.0 N B:GLY15 4.7 46.6 1.0 N B:ASP14 4.8 48.1 1.0 C B:GLY10 5.0 38.6 1.0 O B:GLY10 5.0 40.8 1.0

A.M.El-Araby, E.Jimenez-Faraco, R.Feltzer, J.M.Martin-Garcia, B.R.Karri, B.Ramachandran, C.Kim, J.F.Fisher, J.A.Hermoso, S.Mobashery. Catalytic Process of Anhydro-N-Acetylmuramic Acid Kinase (Anmk) From Pseudomonas Aeruginosa. J.Biol.Chem. 05198 2023.
ISSN: ESSN 1083-351X
PubMed: 37660917
DOI: 10.1016/J.JBC.2023.105198