Magnesium in PDB 8dbo: Human PRPS1-E307A Engineered Mutation with Adp; Hexamer

All present enzymatic activity of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer:
2.7.6.1;

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Magnesium atom in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer (pdb code 8dbo). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer, PDB code: 8dbo:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:22.4 occ:1.00 O A:HOH503 1.9 30.0 1.0 O F:HOH501 1.9 30.0 1.0 NE2 A:HIS130 2.2 20.7 1.0 O F:HOH502 2.3 30.0 1.0 O1A F:ADP401 2.6 35.1 1.0 O3A F:ADP401 2.6 25.7 1.0 CE1 A:HIS130 3.1 20.7 1.0 PA F:ADP401 3.2 27.6 1.0 CD2 A:HIS130 3.3 20.7 1.0 O1B F:ADP401 3.5 27.8 1.0 PB F:ADP401 3.6 32.2 1.0 NZ A:LYS99 3.9 21.9 1.0 O5' F:ADP401 4.1 27.3 1.0 O2B F:ADP401 4.1 34.6 1.0 OD1 A:ASP98 4.2 20.6 1.0 C5' F:ADP401 4.3 23.6 1.0 ND1 A:HIS130 4.3 20.7 1.0 O2A F:ADP401 4.3 20.9 1.0 CG A:HIS130 4.4 20.7 1.0 OD2 A:ASP98 4.4 20.6 1.0 CE A:LYS99 4.5 21.9 1.0 O A:ARG96 4.7 21.9 1.0 CG A:ASP98 4.7 20.6 1.0 O3B F:ADP401 4.9 41.6 1.0

Magnesium binding site 2 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg404 b:30.0 occ:1.00 OD2 A:ASP171 2.6 25.8 1.0 O2A F:ADP401 3.5 20.9 1.0 CG A:ASP171 3.7 25.8 1.0 OD1 A:ASP171 4.2 25.8 1.0 CE1 A:HIS130 4.3 20.7 1.0 OD2 A:ASP220 4.4 25.8 1.0 O1A F:ADP401 4.4 35.1 1.0 OD1 A:ASP221 4.4 25.3 1.0 PA F:ADP401 4.5 27.6 1.0 OD1 A:ASP220 4.6 25.8 1.0 O F:HOH502 4.7 30.0 1.0 CB A:ASP171 4.9 25.8 1.0 CG A:ASP220 4.9 25.8 1.0

Magnesium binding site 3 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg403 b:22.4 occ:1.00 O B:HOH503 1.9 30.0 1.0 O C:HOH501 1.9 30.0 1.0 NE2 B:HIS130 2.2 20.7 1.0 O C:HOH502 2.3 30.0 1.0 O2A C:ADP401 2.6 35.1 1.0 O3A C:ADP401 2.6 25.7 1.0 CE1 B:HIS130 3.1 20.7 1.0 PA C:ADP401 3.2 27.6 1.0 CD2 B:HIS130 3.3 20.7 1.0 O3B C:ADP401 3.5 27.8 1.0 PB C:ADP401 3.6 32.2 1.0 NZ B:LYS99 3.9 21.9 1.0 O5' C:ADP401 4.1 27.3 1.0 O1B C:ADP401 4.1 34.6 1.0 OD1 B:ASP98 4.2 20.6 1.0 C5' C:ADP401 4.3 23.6 1.0 ND1 B:HIS130 4.3 20.7 1.0 O1A C:ADP401 4.3 20.9 1.0 CG B:HIS130 4.4 20.7 1.0 OD2 B:ASP98 4.4 20.6 1.0 CE B:LYS99 4.5 21.9 1.0 O B:ARG96 4.7 21.9 1.0 CG B:ASP98 4.7 20.6 1.0 O2B C:ADP401 4.9 41.6 1.0

Magnesium binding site 4 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg404 b:30.0 occ:1.00 OD2 B:ASP171 2.6 25.8 1.0 O1A C:ADP401 3.5 20.9 1.0 CG B:ASP171 3.7 25.8 1.0 OD1 B:ASP171 4.2 25.8 1.0 CE1 B:HIS130 4.3 20.7 1.0 OD2 B:ASP220 4.4 25.8 1.0 O2A C:ADP401 4.4 35.1 1.0 OD1 B:ASP221 4.4 25.3 1.0 PA C:ADP401 4.5 27.6 1.0 OD1 B:ASP220 4.6 25.8 1.0 O C:HOH502 4.7 30.0 1.0 CB B:ASP171 4.9 25.8 1.0 CG B:ASP220 4.9 25.8 1.0

Magnesium binding site 5 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg404 b:22.4 occ:1.00 O C:HOH503 1.9 30.0 1.0 O B:HOH501 1.9 30.0 1.0 NE2 C:HIS130 2.2 20.7 1.0 O B:HOH502 2.3 30.0 1.0 O1A B:ADP405 2.6 35.1 1.0 O3A B:ADP405 2.6 25.7 1.0 CE1 C:HIS130 3.1 20.7 1.0 PA B:ADP405 3.2 27.6 1.0 CD2 C:HIS130 3.3 20.7 1.0 O1B B:ADP405 3.5 27.8 1.0 PB B:ADP405 3.6 32.2 1.0 NZ C:LYS99 3.9 21.9 1.0 O5' B:ADP405 4.1 27.3 1.0 O2B B:ADP405 4.1 34.6 1.0 OD1 C:ASP98 4.2 20.6 1.0 C5' B:ADP405 4.3 23.6 1.0 ND1 C:HIS130 4.3 20.7 1.0 O2A B:ADP405 4.3 20.9 1.0 CG C:HIS130 4.4 20.7 1.0 OD2 C:ASP98 4.4 20.6 1.0 CE C:LYS99 4.5 21.9 1.0 O C:ARG96 4.7 21.9 1.0 CG C:ASP98 4.7 20.6 1.0 O3B B:ADP405 4.9 41.6 1.0

Magnesium binding site 6 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg405 b:30.0 occ:1.00 OD2 C:ASP171 2.6 25.8 1.0 O2A B:ADP405 3.5 20.9 1.0 CG C:ASP171 3.7 25.8 1.0 OD1 C:ASP171 4.2 25.8 1.0 CE1 C:HIS130 4.3 20.7 1.0 OD2 C:ASP220 4.4 25.8 1.0 O1A B:ADP405 4.4 35.1 1.0 OD1 C:ASP221 4.4 25.3 1.0 PA B:ADP405 4.5 27.6 1.0 OD1 C:ASP220 4.6 25.8 1.0 O B:HOH502 4.7 30.0 1.0 CB C:ASP171 4.9 25.8 1.0 CG C:ASP220 4.9 25.8 1.0

Magnesium binding site 7 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg403 b:22.4 occ:1.00 O D:HOH503 1.9 30.0 1.0 O E:HOH501 1.9 30.0 1.0 NE2 D:HIS130 2.2 20.7 1.0 O E:HOH502 2.3 30.0 1.0 O2A E:ADP401 2.6 35.1 1.0 O3A E:ADP401 2.6 25.7 1.0 CE1 D:HIS130 3.1 20.7 1.0 PA E:ADP401 3.2 27.6 1.0 CD2 D:HIS130 3.3 20.7 1.0 O3B E:ADP401 3.5 27.8 1.0 PB E:ADP401 3.6 32.2 1.0 NZ D:LYS99 3.9 21.9 1.0 O5' E:ADP401 4.1 27.3 1.0 O1B E:ADP401 4.1 34.6 1.0 OD1 D:ASP98 4.2 20.6 1.0 C5' E:ADP401 4.3 23.6 1.0 ND1 D:HIS130 4.3 20.7 1.0 O1A E:ADP401 4.3 20.9 1.0 CG D:HIS130 4.4 20.7 1.0 OD2 D:ASP98 4.4 20.6 1.0 CE D:LYS99 4.5 21.9 1.0 O D:ARG96 4.7 21.9 1.0 CG D:ASP98 4.7 20.6 1.0 O2B E:ADP401 4.9 41.6 1.0

Magnesium binding site 8 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg404 b:30.0 occ:1.00 OD2 D:ASP171 2.6 25.8 1.0 O1A E:ADP401 3.5 20.9 1.0 CG D:ASP171 3.7 25.8 1.0 OD1 D:ASP171 4.2 25.8 1.0 CE1 D:HIS130 4.3 20.7 1.0 OD2 D:ASP220 4.4 25.8 1.0 O2A E:ADP401 4.4 35.1 1.0 OD1 D:ASP221 4.4 25.3 1.0 PA E:ADP401 4.5 27.6 1.0 OD1 D:ASP220 4.6 25.8 1.0 O E:HOH502 4.7 30.0 1.0 CB D:ASP171 4.9 25.8 1.0 CG D:ASP220 4.9 25.8 1.0

Magnesium binding site 9 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg404 b:22.4 occ:1.00 O E:HOH503 1.9 30.0 1.0 O D:HOH501 1.9 30.0 1.0 NE2 E:HIS130 2.2 20.7 1.0 O D:HOH502 2.3 30.0 1.0 O1A D:ADP405 2.6 35.1 1.0 O3A D:ADP405 2.6 25.7 1.0 CE1 E:HIS130 3.1 20.7 1.0 PA D:ADP405 3.2 27.6 1.0 CD2 E:HIS130 3.3 20.7 1.0 O1B D:ADP405 3.5 27.8 1.0 PB D:ADP405 3.6 32.2 1.0 NZ E:LYS99 3.9 21.9 1.0 O5' D:ADP405 4.1 27.3 1.0 O2B D:ADP405 4.1 34.6 1.0 OD1 E:ASP98 4.2 20.6 1.0 C5' D:ADP405 4.3 23.6 1.0 ND1 E:HIS130 4.3 20.7 1.0 O2A D:ADP405 4.3 20.9 1.0 CG E:HIS130 4.4 20.7 1.0 OD2 E:ASP98 4.4 20.6 1.0 CE E:LYS99 4.5 21.9 1.0 O E:ARG96 4.7 21.9 1.0 CG E:ASP98 4.7 20.6 1.0 O3B D:ADP405 4.9 41.6 1.0

Magnesium binding site 10 out of 12 in the Human PRPS1-E307A Engineered Mutation with Adp; Hexamer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Human PRPS1-E307A Engineered Mutation with Adp; Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg405 b:30.0 occ:1.00 OD2 E:ASP171 2.6 25.8 1.0 O2A D:ADP405 3.5 20.9 1.0 CG E:ASP171 3.7 25.8 1.0 OD1 E:ASP171 4.2 25.8 1.0 CE1 E:HIS130 4.3 20.7 1.0 OD2 E:ASP220 4.4 25.8 1.0 O1A D:ADP405 4.4 35.1 1.0 OD1 E:ASP221 4.4 25.3 1.0 PA D:ADP405 4.5 27.6 1.0 OD1 E:ASP220 4.6 25.8 1.0 O D:HOH502 4.7 30.0 1.0 CB E:ASP171 4.9 25.8 1.0 CG E:ASP220 4.9 25.8 1.0

K.L.Hvorecny, J.M.Kollman. Human PRPS1 Filaments Stabilize Allosteric Sites to Regulate Activity Nat.Struct.Mol.Biol. 2023.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-023-00921-Z