Magnesium in PDB 8eg7: Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex

All present enzymatic activity of Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex:
2.7.7.6;

The structure of Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex (pdb code 8eg7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex, PDB code: 8eg7:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Pre-Consensus Elemental Paused Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1501 b:33.6 occ:1.00 O3' R:G16 2.0 38.3 1.0 OD1 J:ASP460 2.0 31.6 1.0 OD1 J:ASP464 2.0 37.4 1.0 OD2 J:ASP464 2.4 37.4 1.0 CG J:ASP464 2.5 37.4 1.0 O2' R:G16 2.8 38.3 1.0 OD1 J:ASP462 2.9 38.3 1.0 CG J:ASP460 3.1 31.6 1.0 C3' R:G16 3.1 38.3 1.0 C2' R:G16 3.5 38.3 1.0 OD2 J:ASP460 3.5 31.6 1.0 C4' R:G16 3.7 38.3 1.0 CG J:ASP462 3.8 38.3 1.0 OD2 J:ASP462 3.9 38.3 1.0 CB J:ASP464 4.0 37.4 1.0 NH2 J:ARG425 4.1 29.7 1.0 N J:ASP460 4.1 31.6 1.0 O J:ASP460 4.3 31.6 1.0 CB J:ASP460 4.4 31.6 1.0 C J:ASP460 4.6 31.6 1.0 CA J:ASP460 4.6 31.6 1.0 C5' R:G16 4.7 38.3 1.0 C1' R:G16 4.7 38.3 1.0 CA J:ASP464 4.7 37.4 1.0 N J:ASP464 4.7 37.4 1.0 O4' R:G16 4.8 38.3 1.0 NE J:ARG425 4.9 29.7 1.0 CZ J:ARG425 5.0 29.7 1.0

J.Y.Kang, T.V.Mishanina, Y.Bao, J.Chen, E.Llewellyn, J.Liu, S.A.Darst, R.Landick. An Ensemble of Interconverting Conformations of the Elemental Paused Transcription Complex Creates Regulatory Options. Proc.Natl.Acad.Sci.Usa V. 120 45120 2023.
ISSN: ESSN 1091-6490
PubMed: 36795753
DOI: 10.1073/PNAS.2215945120