Magnesium in PDB 8eos: M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp

All present enzymatic activity of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp:
2.7.7.6;

The structure of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp (pdb code 8eos). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp, PDB code: 8eos:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2001 b:33.0 occ:1.00 O3' R:A30 2.1 58.8 1.0 OD1 D:ASP535 2.3 53.0 1.0 OD1 D:ASP539 2.4 53.2 1.0 CG D:ASP539 3.2 50.7 1.0 OD2 D:ASP539 3.2 53.6 1.0 OD2 D:ASP537 3.4 41.0 1.0 CG D:ASP535 3.4 47.4 1.0 C3' R:A30 3.4 47.0 1.0 O2' R:A30 3.5 55.1 1.0 OD1 D:ASP537 3.6 45.5 1.0 CG D:ASP537 3.8 37.8 1.0 OD2 D:ASP535 3.8 49.6 1.0 O D:ASP535 3.8 45.2 1.0 C2' R:A30 4.0 41.9 1.0 C4' R:A30 4.0 43.2 1.0 H3 C:2TM1201 4.2 53.5 1.0 N D:ASP535 4.5 35.2 1.0 C D:ASP535 4.5 43.2 1.0 CB D:ASP539 4.6 51.2 1.0 CB D:ASP535 4.6 37.1 1.0 C5' R:A30 4.7 44.6 1.0 CA D:ASP535 4.8 37.0 1.0 H17 C:2TM1201 4.8 52.6 1.0 H1 C:2TM1201 4.8 52.8 1.0 C4' C:2TM1201 5.0 53.1 1.0 NH2 D:ARG500 5.0 55.5 1.0

Magnesium binding site 2 out of 2 in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2002 b:68.2 occ:1.00 O3G C:2TM1201 1.8 58.2 1.0 O1B C:2TM1201 1.9 64.3 1.0 PB C:2TM1201 2.8 72.0 1.0 PG C:2TM1201 2.9 79.0 1.0 OD2 D:ASP535 3.0 49.6 1.0 H17 C:2TM1201 3.1 52.6 1.0 O3B C:2TM1201 3.2 65.4 1.0 C1 C:2TM1201 3.5 48.5 1.0 O1G C:2TM1201 3.6 55.6 1.0 H18 C:2TM1201 3.9 51.6 1.0 CG D:ASP535 3.9 47.4 1.0 O2G C:2TM1201 4.2 62.1 1.0 O2B C:2TM1201 4.2 53.4 1.0 CB D:ASP535 4.5 37.1 1.0 OD1 D:ASP535 4.8 53.0 1.0

R.K.Vishwakarma, M.Z.Qayyum, P.Babitzke, K.S.Murakami. Structural Basis of Nusg-Dependent Rna Polymerase Transcription Pausing in Bacteria To Be Published.