Magnesium in PDB 8frg: Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43

All present enzymatic activity of Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43:
3.2.1.14;

The structure of Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43, PDB code: 8frg was solved by R.E.Diaz, J.S.Fraser, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.59 / 1.74
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	92.893, 105.041, 70.812, 90, 90, 90
R / Rfree (%)	16.6 / 20.8

The binding sites of Magnesium atom in the Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43 (pdb code 8frg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43, PDB code: 8frg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:29.8 occ:1.00 O A:HOH861 1.9 38.8 1.0 O A:HOH558 2.1 24.2 1.0 O A:HOH897 2.3 35.0 1.0 O A:HOH648 2.3 37.0 1.0 HA A:TYR34 3.9 15.6 1.0 HA A:PRO36 4.1 19.4 1.0 O A:TYR34 4.2 11.9 1.0 O A:ARG35 4.2 11.9 1.0 O A:HOH876 4.2 32.1 1.0 O A:HOH711 4.3 18.2 1.0 O A:HOH668 4.4 20.1 1.0 O A:GLN33 4.4 13.4 1.0 C A:TYR34 4.5 11.8 1.0 CA A:TYR34 4.7 13.0 1.0 HB2 A:SER40 4.8 14.1 1.0 C A:ARG35 4.9 14.8 1.0 HD1 A:TYR34 4.9 18.1 1.0

Magnesium binding site 2 out of 2 in the Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Mouse Acidic Mammalian Chitinase, Catalytic Domain in Complex with N, N'-Diacetylchitobiose at pH 5.43 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:29.3 occ:1.00 O A:HOH536 2.1 29.7 1.0 O A:HOH822 2.2 36.9 1.0 O A:HOH578 2.4 29.2 1.0 HD21 A:ASN275 3.8 47.9 1.0 HD22 A:ASN275 4.0 47.9 1.0 OD2 A:ASP278 4.2 22.0 1.0 ND2 A:ASN275 4.2 39.9 1.0 OD1 A:ASP278 4.3 24.8 1.0 O A:HOH766 4.3 46.4 1.0 O A:HOH741 4.4 31.7 1.0 CG A:ASP278 4.7 22.1 1.0

R.E.Diaz, J.S.Fraser. Structural Characterization of Ligand Binding and pH-Specific Enzymatic Activity of Mouse Acidic Mammalian Chitinase To Be Published.