Magnesium in PDB 8hx8: Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate

All present enzymatic activity of Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate:
2.6.1.85;

The structure of Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate, PDB code: 8hx8 was solved by Y.Nakamichi, M.Watanabe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.50 / 2.55
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	98.334, 172.848, 161.054, 90, 90, 90
R / Rfree (%)	20.6 / 26.8

The binding sites of Magnesium atom in the Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate (pdb code 8hx8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate, PDB code: 8hx8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg703 b:88.0 occ:1.00 O A:HOH801 2.5 57.9 1.0 O A:SER174 2.5 69.5 1.0 O A:HOH802 2.6 68.5 1.0 O A:ILE172 2.8 80.6 1.0 C A:ILE172 3.7 78.2 1.0 C A:SER174 3.7 70.5 1.0 OD1 A:ASP175 4.2 73.2 1.0 N A:SER174 4.2 69.0 1.0 CA A:ILE172 4.2 77.5 1.0 O A:SER171 4.3 73.4 1.0 CD1 A:ILE172 4.5 78.7 1.0 C A:GLY173 4.6 70.0 1.0 CA A:SER174 4.6 69.1 1.0 N A:GLY173 4.6 74.3 1.0 OH A:TYR19 4.7 70.3 1.0 N A:ASP175 4.7 70.9 1.0 CA A:ASP175 4.8 68.6 1.0 CG1 A:ILE172 4.9 80.4 1.0 CA A:GLY173 4.9 72.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of 4-Amino-4-Deoxychorismate Synthase From Streptomyces Venezuelae Co-Crystallized with Chorismate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg704 b:89.0 occ:1.00 OE2 A:GLU528 2.7 88.3 1.0 OE2 A:GLU662 2.8 82.4 1.0 OE1 A:GLU662 3.0 78.5 1.0 O A:ALA652 3.1 80.7 1.0 OE1 A:GLU528 3.2 82.1 1.0 CD A:GLU662 3.3 79.2 1.0 CD A:GLU528 3.3 83.9 1.0 OE1 A:GLU665 3.4 84.2 1.0 NZ A:LYS524 3.4 89.5 1.0 OE2 A:GLU665 3.7 78.7 1.0 N A:ALA652 3.9 80.5 1.0 CD A:GLU665 3.9 83.3 1.0 C A:ALA652 4.0 79.9 1.0 CB A:ALA652 4.0 80.8 1.0 CA A:ALA652 4.2 80.5 1.0 CE A:LYS524 4.2 89.5 1.0 CG A:GLU528 4.7 78.6 1.0 CG A:GLU662 4.8 82.3 1.0 C A:GLY651 4.9 79.5 1.0 OG A:SER657 5.0 89.1 1.0

Y.Nakamichi, J.Kobayashi, K.Toyoda, M.Suda, K.Hiraga, M.Inui, M.Watanabe. Structural Basis For the Allosteric Pathway of 4-Amino-4-Deoxychorismate Synthase. Acta Crystallogr D Struct V. 79 895 2023BIOL.
ISSN: ISSN 2059-7983
PubMed: 37712435
DOI: 10.1107/S2059798323006320