Atomistry » Magnesium » PDB 8om0-8oum » 8ooo
Atomistry »
  Magnesium »
    PDB 8om0-8oum »
      8ooo »

Magnesium in PDB 8ooo: Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution

Enzymatic activity of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution

All present enzymatic activity of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution:
6.3.1.2;

Protein crystallography data

The structure of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution, PDB code: 8ooo was solved by M.-C.Mueller, T.Wagner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.88 / 2.15
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 112.337, 131.773, 131.507, 60.04, 87.72, 67.34
R / Rfree (%) 18.2 / 21.4

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution (pdb code 8ooo). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution, PDB code: 8ooo:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in 8ooo

Go back to Magnesium Binding Sites List in 8ooo
Magnesium binding site 1 out of 3 in the Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg505

b:50.0
occ:0.50
 O H:HOH921 2.8 57.3 1.0
OD2 B:ASP203 3.0 38.5 1.0
NE2 B:HIS192 3.0 24.7 1.0
O B:HOH651 3.0 36.8 1.0
O2G B:ATP506 3.1 64.2 1.0
OE2 B:GLU201 3.6 46.0 1.0
CD2 B:HIS192 3.7 23.5 1.0
CD B:GLU201 3.9 44.3 1.0
CE1 B:HIS192 4.0 25.1 1.0
CG B:ASP203 4.0 32.2 1.0
O1A B:ATP506 4.1 55.4 1.0
OE1 B:GLU201 4.2 48.4 1.0
PG B:ATP506 4.4 63.8 1.0
OD1 B:ASP203 4.4 33.4 1.0
OE2 B:GLU137 4.6 47.5 1.0
O1G B:ATP506 4.7 63.6 1.0
O2B B:ATP506 4.8 59.7 1.0
CG B:GLU201 4.8 37.7 1.0
CG B:HIS192 4.9 24.4 1.0

Magnesium binding site 2 out of 3 in 8ooo

Go back to Magnesium Binding Sites List in 8ooo
Magnesium binding site 2 out of 3 in the Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Mg602

b:50.4
occ:0.50
 O2G E:ATP604 2.6 66.9 1.0
OD2 E:ASP203 2.8 39.1 1.0
O D:HOH822 2.9 60.5 1.0
O E:HOH743 3.0 46.2 1.0
OE2 E:GLU201 3.4 44.2 1.0
O1A E:ATP604 3.6 58.2 1.0
NE2 E:HIS192 3.6 29.8 1.0
CG E:ASP203 3.9 33.6 1.0
CD E:GLU201 4.0 41.6 1.0
PG E:ATP604 4.0 66.3 1.0
OE2 E:GLU137 4.0 51.5 1.0
O2B E:ATP604 4.2 62.5 1.0
OE1 E:GLU201 4.3 45.4 1.0
OD1 E:ASP203 4.3 34.1 1.0
CD2 E:HIS192 4.4 27.9 1.0
O1G E:ATP604 4.4 66.3 1.0
CE1 E:HIS192 4.6 29.9 1.0
O3B E:ATP604 4.8 64.3 1.0
O3G E:ATP604 4.9 66.5 1.0
CD E:GLU137 5.0 46.7 1.0
CG E:GLU201 5.0 33.2 1.0

Magnesium binding site 3 out of 3 in 8ooo

Go back to Magnesium Binding Sites List in 8ooo
Magnesium binding site 3 out of 3 in the Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Glutamine Synthetase From Methanothermococcus Thermolithotrophicus in Complex with 2-Oxoglutarate and Mgatp at 2.15 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
K:Mg502

b:31.7
occ:0.50
 O2G K:ATP504 2.2 67.0 1.0
OD2 K:ASP203 2.4 46.9 1.0
O1A K:ATP504 2.8 59.0 1.0
O I:HOH831 3.0 44.2 1.0
OE2 K:GLU137 3.0 52.5 1.0
OE2 K:GLU201 3.1 38.1 1.0
CG K:ASP203 3.4 42.6 1.0
PG K:ATP504 3.6 66.0 1.0
O K:HOH688 3.7 43.4 1.0
OD1 K:ASP203 3.8 44.9 1.0
O K:HOH670 3.8 43.6 1.0
O2B K:ATP504 3.9 63.5 1.0
CD K:GLU201 4.0 37.5 1.0
O1G K:ATP504 4.0 65.8 1.0
CD K:GLU137 4.1 46.2 1.0
PA K:ATP504 4.3 59.1 1.0
O3B K:ATP504 4.3 64.3 1.0
NE2 K:HIS192 4.4 30.4 1.0
OE1 K:GLU201 4.4 36.9 1.0
PB K:ATP504 4.6 62.6 1.0
O3G K:ATP504 4.6 66.0 1.0
OE1 K:GLU137 4.7 47.0 1.0
CB K:ASP203 4.7 33.5 1.0
O K:HOH757 4.8 52.6 1.0
O3A K:ATP504 4.9 61.0 1.0

Reference:

M.C.Muller, O.N.Lemaire, J.M.Kurth, C.U.Welte, T.Wagner. Differences in Regulation Mechanisms of Glutamine Synthetases From Methanogenic Archaea Unveiled By Structural Investigations. Commun Biol V. 7 111 2024.
ISSN: ESSN 2399-3642
PubMed: 38243071
DOI: 10.1038/S42003-023-05726-W
Page generated: Fri Oct 4 15:00:19 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy