Magnesium in PDB 8ruc: Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate

All present enzymatic activity of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate:
4.1.1.39;

The structure of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate, PDB code: 8ruc was solved by I.Andersson, S.Knight, C.-I.Branden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.00 / 1.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	157.200, 157.200, 201.300, 90.00, 90.00, 90.00
R / Rfree (%)	21.1 / n/a

The binding sites of Magnesium atom in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate (pdb code 8ruc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate, PDB code: 8ruc:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg476 b:15.1 occ:1.00 OE1 A:GLU204 2.3 8.8 1.0 OD1 A:ASP203 2.3 12.9 1.0 O6 A:CAP477 2.3 13.5 1.0 OQ2 A:KCX201 2.3 10.8 1.0 O2 A:CAP477 2.4 15.2 1.0 O3 A:CAP477 2.4 13.9 1.0 C2 A:CAP477 2.9 16.5 1.0 C A:CAP477 2.9 16.4 1.0 C3 A:CAP477 3.2 14.0 1.0 CD A:GLU204 3.3 12.4 1.0 CX A:KCX201 3.3 14.6 1.0 CG A:ASP203 3.3 11.8 1.0 OQ1 A:KCX201 3.5 12.3 1.0 OE2 A:GLU204 3.7 13.9 1.0 NZ A:LYS177 3.7 10.3 1.0 N A:GLU204 3.8 9.0 1.0 CG2 A:THR173 3.8 10.0 1.0 OD2 A:ASP203 3.9 9.9 1.0 NZ A:LYS175 4.0 6.3 1.0 NE2 A:HIS294 4.0 7.4 1.0 O7 A:CAP477 4.1 14.1 1.0 CA A:ASP203 4.2 10.6 1.0 CB A:ASP203 4.3 7.9 1.0 C1 A:CAP477 4.4 12.1 1.0 C4 A:CAP477 4.4 13.0 1.0 OG1 A:THR173 4.4 7.7 1.0 CG A:GLU204 4.5 12.4 1.0 NZ A:KCX201 4.5 6.8 1.0 C A:ASP203 4.5 9.9 1.0 CB A:GLU204 4.6 9.6 1.0 CB A:THR173 4.6 10.1 1.0 CD2 A:HIS294 4.6 5.8 1.0 O1 A:CAP477 4.8 12.2 1.0 CA A:GLU204 4.8 8.6 1.0 C5 A:CAP477 4.9 11.0 1.0

Magnesium binding site 2 out of 4 in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg476 b:15.1 occ:1.00 OD1 C:ASP203 2.3 11.9 1.0 OE1 C:GLU204 2.3 11.9 1.0 OQ2 C:KCX201 2.3 11.3 1.0 O3 C:CAP477 2.3 11.2 1.0 O6 C:CAP477 2.4 14.5 1.0 O2 C:CAP477 2.4 13.0 1.0 C2 C:CAP477 2.8 15.2 1.0 C C:CAP477 2.9 13.6 1.0 C3 C:CAP477 3.1 13.3 1.0 CX C:KCX201 3.2 11.7 1.0 CD C:GLU204 3.2 13.5 1.0 OQ1 C:KCX201 3.3 11.1 1.0 CG C:ASP203 3.4 13.6 1.0 OE2 C:GLU204 3.6 12.6 1.0 NZ C:LYS177 3.8 8.2 1.0 N C:GLU204 3.9 6.1 1.0 NZ C:LYS175 4.0 5.9 1.0 CG2 C:THR173 4.0 5.6 1.0 NE2 C:HIS294 4.0 9.8 1.0 O7 C:CAP477 4.1 12.8 1.0 OD2 C:ASP203 4.1 18.3 1.0 CA C:ASP203 4.2 8.1 1.0 OG1 C:THR173 4.3 10.8 1.0 C4 C:CAP477 4.3 14.4 1.0 C1 C:CAP477 4.3 13.9 1.0 CB C:ASP203 4.4 10.1 1.0 NZ C:KCX201 4.4 8.9 1.0 CG C:GLU204 4.5 3.9 1.0 C C:ASP203 4.6 6.9 1.0 CB C:GLU204 4.6 5.9 1.0 CD2 C:HIS294 4.6 8.1 1.0 CB C:THR173 4.7 10.6 1.0 C5 C:CAP477 4.8 11.9 1.0 O1 C:CAP477 4.9 13.8 1.0 CA C:GLU204 4.9 7.2 1.0 CE1 C:HIS294 4.9 10.9 1.0

Magnesium binding site 3 out of 4 in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg476 b:15.4 occ:1.00 OQ2 E:KCX201 2.3 10.9 1.0 OD1 E:ASP203 2.3 10.9 1.0 OE1 E:GLU204 2.3 12.4 1.0 O6 E:CAP477 2.3 14.6 1.0 O2 E:CAP477 2.4 12.7 1.0 O3 E:CAP477 2.4 15.4 1.0 C2 E:CAP477 3.0 14.4 1.0 C E:CAP477 3.0 14.3 1.0 C3 E:CAP477 3.2 13.9 1.0 CX E:KCX201 3.2 13.2 1.0 CD E:GLU204 3.3 12.6 1.0 CG E:ASP203 3.3 11.1 1.0 OQ1 E:KCX201 3.3 10.0 1.0 OE2 E:GLU204 3.6 9.2 1.0 NZ E:LYS177 3.9 11.3 1.0 N E:GLU204 3.9 5.6 1.0 OD2 E:ASP203 3.9 9.7 1.0 NE2 E:HIS294 4.0 6.0 1.0 CG2 E:THR173 4.0 8.7 1.0 NZ E:LYS175 4.1 5.5 1.0 CA E:ASP203 4.2 8.9 1.0 O7 E:CAP477 4.2 13.4 1.0 CB E:ASP203 4.3 7.5 1.0 OG1 E:THR173 4.4 10.9 1.0 C4 E:CAP477 4.4 14.1 1.0 C1 E:CAP477 4.4 10.7 1.0 NZ E:KCX201 4.4 8.2 1.0 CG E:GLU204 4.5 8.2 1.0 C E:ASP203 4.5 9.2 1.0 CB E:GLU204 4.6 9.4 1.0 CD2 E:HIS294 4.7 5.9 1.0 CB E:THR173 4.7 8.8 1.0 O1 E:CAP477 4.8 12.4 1.0 C5 E:CAP477 4.8 10.1 1.0 CE1 E:HIS294 4.8 5.8 1.0 CA E:GLU204 4.9 8.1 1.0

Magnesium binding site 4 out of 4 in the Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Activated Spinach Rubisco Complexed with 2-Carboxyarabinitol Bisphosphate within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg476 b:14.2 occ:1.00 OE1 G:GLU204 2.3 12.5 1.0 OD1 G:ASP203 2.3 15.3 1.0 OQ2 G:KCX201 2.3 14.6 1.0 O2 G:CAP477 2.3 13.8 1.0 O6 G:CAP477 2.4 10.9 1.0 O3 G:CAP477 2.4 9.5 1.0 C2 G:CAP477 2.8 12.9 1.0 C G:CAP477 2.9 11.5 1.0 C3 G:CAP477 3.1 13.6 1.0 CX G:KCX201 3.2 14.5 1.0 OQ1 G:KCX201 3.3 12.2 1.0 CD G:GLU204 3.3 13.6 1.0 CG G:ASP203 3.4 11.4 1.0 OE2 G:GLU204 3.8 13.8 1.0 N G:GLU204 3.8 5.1 1.0 NZ G:LYS177 4.0 12.0 1.0 NE2 G:HIS294 4.0 8.5 1.0 CG2 G:THR173 4.0 10.2 1.0 OD2 G:ASP203 4.0 11.1 1.0 O7 G:CAP477 4.1 10.7 1.0 NZ G:LYS175 4.1 14.0 1.0 CA G:ASP203 4.2 8.8 1.0 C4 G:CAP477 4.2 14.1 1.0 OG1 G:THR173 4.3 12.3 1.0 C1 G:CAP477 4.3 14.6 1.0 CB G:ASP203 4.4 9.0 1.0 NZ G:KCX201 4.5 11.7 1.0 CD2 G:HIS294 4.5 7.7 1.0 CG G:GLU204 4.5 6.3 1.0 C G:ASP203 4.5 8.2 1.0 CB G:GLU204 4.6 6.3 1.0 CB G:THR173 4.7 10.8 1.0 C5 G:CAP477 4.7 11.2 1.0 O1 G:CAP477 4.8 13.3 1.0 CA G:GLU204 4.8 6.7 1.0 CE1 G:HIS294 4.9 5.9 1.0

I.Andersson, I.Andersson, S.Knight, C.-I.Branden. N/A N/A.
ISSN: ISSN 0022-2836
PubMed: 8648644
DOI: 10.1006/JMBI.1996.0310