Magnesium in PDB 8ucf: Thermophilic Rna Ligase From Palaeococcus Pacificus K238G

All present enzymatic activity of Thermophilic Rna Ligase From Palaeococcus Pacificus K238G:
6.5.1.3;

The structure of Thermophilic Rna Ligase From Palaeococcus Pacificus K238G, PDB code: 8ucf was solved by M.D.Rousseau, J.L.Hicks, T.Oulavallickal, A.Williamson, V.L.Arcus, M.W.Patrick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.57 / 1.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.95, 96.859, 139.454, 90, 90, 90
R / Rfree (%)	17.1 / 19.7

The binding sites of Magnesium atom in the Thermophilic Rna Ligase From Palaeococcus Pacificus K238G (pdb code 8ucf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Thermophilic Rna Ligase From Palaeococcus Pacificus K238G, PDB code: 8ucf:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Thermophilic Rna Ligase From Palaeococcus Pacificus K238G


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermophilic Rna Ligase From Palaeococcus Pacificus K238G within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg407 b:13.2 occ:1.00 OD1 A:ASP248 2.0 12.1 1.0 O A:HOH560 2.0 11.8 1.0 O A:HOH559 2.1 11.1 1.0 OE2 A:GLU155 2.1 12.6 1.0 OD2 A:ASP94 2.1 12.2 1.0 O A:HOH519 2.2 12.5 1.0 CD A:GLU155 3.0 13.2 1.0 CG A:ASP94 3.1 14.4 1.0 CG A:ASP248 3.1 12.2 1.0 OD1 A:ASP94 3.4 12.3 1.0 OE1 A:GLU155 3.4 13.7 1.0 OD2 A:ASP248 3.5 11.1 1.0 O A:HOH689 3.8 39.1 1.0 O A:HOH575 3.8 14.5 1.0 O A:HOH705 3.8 36.9 1.0 O A:HOH596 3.9 18.6 1.0 OE2 A:GLU224 4.2 15.3 1.0 O A:ALA244 4.2 13.2 1.0 CG A:GLU155 4.3 13.0 1.0 CB A:ASP248 4.4 8.9 1.0 CB A:ASP94 4.4 11.1 1.0 CD A:GLU224 4.7 15.7 1.0 CA A:ASP248 4.7 8.2 1.0 N A:ASP248 4.7 7.5 1.0 CB A:ALA244 4.8 10.8 1.0 CA A:ALA244 4.9 11.6 1.0 CG A:GLU224 4.9 8.1 1.0 C A:ALA244 4.9 13.4 1.0

Magnesium binding site 2 out of 2 in the Thermophilic Rna Ligase From Palaeococcus Pacificus K238G


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Thermophilic Rna Ligase From Palaeococcus Pacificus K238G within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg408 b:38.7 occ:1.00 O A:HOH509 2.0 24.2 1.0 O A:HOH537 2.1 20.3 1.0 O A:HOH531 2.1 16.0 1.0 O A:HOH685 2.2 47.9 1.0 O A:HOH715 2.5 42.1 1.0 NZ A:LYS92 2.6 20.0 1.0 CE A:LYS92 3.5 26.2 1.0 OE2 A:GLU144 3.6 23.5 1.0 O A:GLY95 3.8 14.1 1.0 OE1 A:GLU224 4.2 11.6 1.0 OE2 A:GLU224 4.2 15.3 1.0 OD1 A:ASP94 4.4 12.3 1.0 O A:HOH659 4.4 30.2 1.0 O A:VAL93 4.5 11.1 1.0 N A:GLY95 4.6 10.9 1.0 CD A:GLU144 4.6 16.8 1.0 O A:HOH510 4.7 40.3 1.0 CD A:GLU224 4.7 15.7 1.0 C A:GLY95 4.8 14.1 1.0 CA A:ASP94 4.9 12.3 1.0 CD A:LYS92 4.9 25.0 1.0 OE1 A:GLU144 5.0 25.1 1.0

M.D.Rousseau, J.L.Hicks, T.Oulavallickal, A.Williamson, V.L.Arcus, M.W.Patrick. Characterisation and Engineering of A Thermophilic Rna Ligase From Palaeococcus Pacificus To Be Published.