Magnesium in PDB 8vsl: Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214)

All present enzymatic activity of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214):
2.7.11.1;

The structure of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214), PDB code: 8vsl was solved by H.R.Vickery, J.M.Virta, M.Pennings, M.Konstantinidou, M.Van Den Oetelaar, R.J.Neitz, C.Ottmann, L.Brunsveld, M.R.Arkin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	66.60 / 1.42
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.518, 112.806, 62.713, 90, 90, 90
R / Rfree (%)	18.2 / 20.8

The structure of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Magnesium atom in the Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) (pdb code 8vsl). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214), PDB code: 8vsl:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:21.7 occ:1.00 O A:HOH404 1.8 27.9 1.0 O A:HOH421 2.2 26.8 1.0 O A:HOH613 2.4 32.2 1.0 OE2 A:GLU89 2.4 15.0 1.0 CD A:GLU89 3.5 10.5 1.0 O A:HOH497 4.1 26.8 1.0 CG A:GLU89 4.2 10.3 1.0 O A:HOH591 4.3 30.2 1.0 NH1 A:ARG85 4.4 13.9 1.0 OE1 A:GLU89 4.4 11.7 1.0 OE1 A:GLN93 4.6 11.5 1.0 CG A:GLU86 4.6 14.9 1.0 NE2 A:GLN93 4.8 13.7 1.0 CB A:GLU89 4.8 9.7 1.0 OG1 A:THR90 5.0 14.4 1.0

Magnesium binding site 2 out of 3 in the Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:16.9 occ:1.00 O A:HOH501 2.4 29.8 1.0 OE1 A:GLU75 2.5 33.9 1.0 O A:HOH536 2.5 35.9 1.0 CD A:GLU75 3.8 32.0 1.0 CG A:GLU75 4.6 35.3 1.0 CB A:GLU75 4.6 34.2 1.0 OE2 A:GLU75 4.6 37.6 1.0 O A:GLY73 4.7 46.3 1.0

Magnesium binding site 3 out of 3 in the Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg304 b:8.6 occ:0.50 OE1 A:GLU2 2.4 13.6 1.0 O A:HOH456 2.5 13.0 1.0 O A:HOH612 2.6 33.4 1.0 CD A:GLU2 3.5 13.7 1.0 OE2 A:GLU2 3.8 12.9 1.0 O A:HOH586 4.3 13.8 1.0 CG A:GLU2 4.7 9.9 1.0 CA A:GLU2 4.8 8.0 1.0 CB A:GLU2 5.0 8.8 1.0 N A:ARG3 5.0 8.1 1.0

H.R.Vickery, J.M.Virta, M.Pennings, M.Konstantinidou, M.Van Den Oetelaar, R.J.Neitz, C.Ottmann, L.Brunsveld, M.R.Arkin. Binary Structure of 14-3-3 Sigma and Araf Phosphopeptide (PS214) To Be Published.