Magnesium in PDB 8y05: Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna

The structure of Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna, PDB code: 8y05 was solved by X.Lin, J.Chen, L.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.01 / 3.10
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	154.764, 154.764, 210.611, 90, 90, 120
R / Rfree (%)	19.7 / 23.5

The binding sites of Magnesium atom in the Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna (pdb code 8y05). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna, PDB code: 8y05:

Magnesium binding site 1 out of 1 in the Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of LBCAS12A in Complex with Crrna and 9NT Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1301 b:12.8 occ:1.00 O A:THR716 2.3 40.0 1.0 OP2 B:A-4 2.6 37.6 1.0 C A:THR716 3.5 42.1 1.0 OD1 A:ASP708 3.8 44.9 1.0 P B:A-4 4.1 36.2 1.0 OP2 B:U-5 4.2 46.0 1.0 CA A:PRO717 4.4 37.4 1.0 N A:PRO717 4.4 36.2 1.0 C A:PRO717 4.4 39.6 1.0 N A:THR716 4.5 45.6 1.0 OP1 B:U-5 4.5 40.5 1.0 CA A:THR716 4.5 41.5 1.0 O5' B:A-4 4.5 34.1 1.0 OD1 A:ASN718 4.5 29.8 1.0 N A:ASN718 4.5 35.0 1.0 P B:U-5 4.6 48.1 1.0 O5' B:U-5 4.7 37.9 1.0 O A:GLY715 4.7 45.6 1.0 CG A:ASP708 4.8 39.2 1.0 C3' B:U-5 4.8 35.5 1.0 N7 B:A-4 4.9 33.9 1.0 C A:GLY715 4.9 48.1 1.0 O A:PRO717 4.9 40.7 1.0 OP2 B:G-3 4.9 39.5 1.0 C8 B:A-4 5.0 34.2 1.0 O3' B:U-5 5.0 32.7 1.0

J.Chen, X.Lin, W.Xiang, Y.Chen, Y.Zhao, L.Huang, L.Liu. Dna Target Binding-Induced Pre-Crrna Processing in Type II and V Crispr-Cas Systems. Nucleic Acids Res. 2024.
ISSN: ESSN 1362-4962
PubMed: 39676682
DOI: 10.1093/NAR/GKAE1241