Magnesium in PDB 8y0b: Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna

All present enzymatic activity of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna:
3.1.21.1; 4.6.1.22;

The structure of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna, PDB code: 8y0b was solved by X.Lin, J.Chen, L.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.21 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	124.729, 124.729, 267.756, 90, 90, 90
R / Rfree (%)	20.3 / 24.8

The binding sites of Magnesium atom in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna (pdb code 8y0b). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna, PDB code: 8y0b:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg101 b:32.4 occ:1.00 O B:HOH203 1.9 54.1 1.0 O B:HOH288 2.0 54.1 1.0 O B:HOH283 2.1 54.1 1.0 O B:HOH241 2.2 54.1 1.0 OP2 B:A-18 3.6 32.6 1.0 O B:HOH260 3.8 35.7 1.0 O A:HOH1605 3.9 32.8 1.0 O4 B:U-17 4.0 29.5 1.0 O A:HOH1858 4.1 54.1 1.0 O B:HOH262 4.3 28.6 1.0 OP2 B:A-12 4.3 29.6 1.0 O B:HOH228 4.4 30.8 1.0 OP2 B:U-13 4.6 33.2 1.0 P B:A-18 4.8 33.6 1.0 N7 B:A-12 4.8 28.3 1.0 O B:HOH209 4.8 38.4 1.0 OP1 B:A-18 4.8 32.8 1.0 C5 B:C-11 4.8 30.1 1.0 O B:HOH290 4.9 54.1 1.0 C4 B:U-17 4.9 28.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 12NT Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1401 b:30.7 occ:1.00 O A:HOH1664 1.8 54.1 1.0 OP2 B:A-4 2.0 33.6 1.0 O A:ARG800 2.1 34.9 1.0 O A:HOH1662 2.2 54.1 1.0 C A:ARG800 3.3 27.3 1.0 P B:A-4 3.5 28.9 1.0 O B:HOH207 4.0 28.2 1.0 O5' B:A-4 4.0 25.8 1.0 N A:ARG800 4.1 24.8 1.0 OP2 B:U-5 4.1 28.7 1.0 N A:PRO801 4.2 32.3 1.0 CA A:PRO801 4.2 30.5 1.0 CA A:ARG800 4.2 33.5 1.0 OD1 A:ASP792 4.3 28.2 1.0 C A:PRO801 4.4 32.8 1.0 C A:GLY799 4.4 30.1 1.0 O A:HOH1658 4.4 37.8 1.0 OP1 B:A-4 4.5 35.9 1.0 O3' B:U-5 4.5 33.2 1.0 OP1 B:U-5 4.6 32.4 1.0 P B:U-5 4.6 28.5 1.0 N A:ASN802 4.7 25.4 1.0 O A:PRO801 4.7 32.1 1.0 C3' B:U-5 4.7 28.5 1.0 OP2 B:G-3 4.7 27.6 1.0 CB A:ARG800 4.7 36.9 1.0 O B:HOH204 4.7 33.4 1.0 CA A:GLY799 4.8 30.4 1.0 O5' B:U-5 4.8 26.8 1.0 C8 B:A-4 4.9 25.6 1.0 O A:GLY799 4.9 27.1 1.0 OD1 A:ASN802 4.9 26.4 1.0 N7 B:A-4 4.9 27.5 1.0

J.Chen, X.Lin, W.Xiang, Y.Chen, Y.Zhao, L.Huang, L.Liu. Dna Target Binding-Induced Pre-Crrna Processing in Type II and V Crispr-Cas Systems. Nucleic Acids Res. 2024.
ISSN: ESSN 1362-4962
PubMed: 39676682
DOI: 10.1093/NAR/GKAE1241