Magnesium in PDB 8y0c: Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna

All present enzymatic activity of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna:
3.1.21.1; 4.6.1.22;

The structure of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna, PDB code: 8y0c was solved by J.Chen, L.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.22 / 3.45
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	124.283, 124.283, 268.523, 90, 90, 90
R / Rfree (%)	21.6 / 28.5

The binding sites of Magnesium atom in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna (pdb code 8y0c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna, PDB code: 8y0c:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg101 b:2.8 occ:1.00 O4 B:U-17 3.6 24.0 1.0 OP2 B:A-12 3.6 31.2 1.0 OP2 B:U-13 3.9 25.7 1.0 OP2 B:A-18 4.1 25.9 1.0 N7 B:A-12 4.5 27.2 1.0 C4 B:U-17 4.6 29.5 1.0 C5 B:U-13 4.7 24.6 1.0 C5 B:C-11 4.9 31.4 1.0 P B:U-13 4.9 22.7 1.0 O5' B:U-13 4.9 29.1 1.0 C8 B:A-12 4.9 28.0 1.0 C6 B:U-13 5.0 25.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of FNCAS12A in Complex with Pre-Crrna and 18NT Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg102 b:9.1 occ:1.00 O A:ARG800 2.5 23.5 1.0 OP2 B:A-4 2.5 22.4 1.0 OP2 B:U-5 3.5 22.1 1.0 OD1 A:ASP792 3.7 25.1 1.0 C A:ARG800 3.8 22.7 1.0 P B:A-4 4.0 16.6 1.0 OD1 A:ASN802 4.1 26.2 1.0 P B:U-5 4.2 23.5 1.0 OP1 B:U-5 4.3 27.0 1.0 O5' B:U-5 4.4 19.0 1.0 O A:GLY799 4.4 27.1 1.0 CA A:PRO801 4.5 22.3 1.0 O5' B:A-4 4.5 24.0 1.0 N A:PRO801 4.6 20.0 1.0 C3' B:U-5 4.6 19.5 1.0 C A:PRO801 4.7 21.1 1.0 CG A:ASP792 4.7 26.6 1.0 N A:ASN802 4.7 18.9 1.0 CA A:ARG800 4.7 21.3 1.0 N7 B:A-4 4.8 27.9 1.0 O3' B:U-5 4.8 16.8 1.0 C6 B:U-5 4.8 25.6 1.0 C5 B:U-5 4.9 24.0 1.0 N A:ARG800 4.9 23.4 1.0 OD2 A:ASP792 4.9 22.5 1.0 C A:GLY799 4.9 27.9 1.0 C8 B:A-4 5.0 26.6 1.0

J.Chen, X.Lin, W.Xiang, Y.Chen, Y.Zhao, L.Huang, L.Liu. Dna Target Binding-Induced Pre-Crrna Processing in Type II and V Crispr-Cas Systems. Nucleic Acids Res. 2024.
ISSN: ESSN 1362-4962
PubMed: 39676682
DOI: 10.1093/NAR/GKAE1241