Magnesium in PDB 8zup: Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5

The structure of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5, PDB code: 8zup was solved by R.Takeda, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.06 / 1.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.696, 62.273, 68.429, 90, 90, 90
R / Rfree (%)	13.9 / 17.3

The binding sites of Magnesium atom in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5 (pdb code 8zup). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5, PDB code: 8zup:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:10.6 occ:0.56 O A:HOH672 2.0 10.9 0.7 O A:HOH698 2.0 19.2 0.6 O A:HOH765 2.0 32.4 1.0 O A:HOH792 2.1 25.9 1.0 O A:HOH806 2.1 26.9 1.0 O A:HOH720 2.1 25.5 1.0 O A:HOH698 2.3 21.2 0.4 O A:HOH672 2.7 19.7 0.3 OG A:SER30 4.1 9.7 1.0 O A:HOH634 4.1 15.0 1.0 O A:HOH675 4.5 38.0 1.0 OE2 A:GLU17 4.6 9.9 0.9 O A:HOH770 4.6 31.5 1.0 CB A:SER30 4.7 8.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:11.2 occ:0.33 O A:HOH785 1.9 15.9 0.7 O A:HOH794 2.0 18.3 1.0 O A:HOH733 2.1 8.7 0.2 O A:HOH869 2.1 18.7 0.4 O A:HOH841 2.2 16.0 1.0 O A:HOH785 2.2 16.8 0.3 O A:HOH869 2.3 10.8 0.6 O A:HOH871 2.4 50.6 1.0 O A:HOH733 3.6 23.9 0.8 O A:HOH754 3.8 30.1 0.7 OD2 A:ASP197 3.9 15.8 1.0 OD1 A:ASP197 4.4 17.6 1.0 CG A:ASP197 4.6 14.7 1.0 NH2 A:ARG80 4.8 21.7 1.0 NH1 A:ARG80 4.8 21.5 1.0

R.Takeda, E.Tsutsumi, K.Okatsu, S.Fukai, K.Takeda. Structural Characterization of Green Fluorescent Protein in the I-State. Sci Rep V. 14 22832 2024.
ISSN: ESSN 2045-2322
PubMed: 39353998
DOI: 10.1038/S41598-024-73696-Y