Magnesium in PDB 8zuq: Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5

The structure of Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5, PDB code: 8zuq was solved by R.Takeda, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.93 / 1.48
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.498, 62.452, 67.783, 90, 90, 90
R / Rfree (%)	15.6 / 19.3

The binding sites of Magnesium atom in the Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5 (pdb code 8zuq). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5, PDB code: 8zuq:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:13.2 occ:0.54 O A:HOH522 2.0 20.8 0.9 O A:HOH550 2.0 18.4 1.0 O A:HOH562 2.1 20.5 1.0 O A:HOH505 2.2 19.9 1.0 O A:HOH488 2.2 27.7 1.0 O A:HOH487 2.3 28.7 0.6 OD1 A:ASN170 2.3 36.3 0.4 O A:HOH487 2.6 23.4 0.4 CG A:ASN170 3.5 10.0 0.4 O A:GLY174 3.9 10.9 1.0 OE2 A:GLU142 4.0 17.0 1.0 CA A:GLY174 4.1 12.1 1.0 CB A:ASN170 4.1 11.8 0.4 OE1 A:GLU142 4.2 14.6 1.0 O A:HOH471 4.2 17.6 0.6 CB A:ASN170 4.2 11.5 0.6 CG A:ASN170 4.3 9.7 0.6 ND2 A:ASN170 4.3 9.1 0.6 C A:GLY174 4.3 11.3 1.0 CD A:GLU142 4.5 14.7 1.0 ND2 A:ASN170 4.5 12.2 0.4 O A:HOH460 4.8 43.0 1.0 O A:SER175 4.8 11.1 1.0 OD1 A:ASN170 4.8 26.8 0.6 O A:ILE171 4.9 10.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the F99S/M153T/V163A/T203I/E222Q Variant of Gfp at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:11.6 occ:0.60 O A:HOH735 2.0 36.2 1.0 O A:HOH752 2.0 27.2 1.0 O A:HOH640 2.1 22.0 1.0 O A:HOH679 2.1 20.0 0.5 O A:HOH769 2.1 28.5 1.0 O A:HOH679 2.1 18.9 0.5 O A:HOH693 2.2 28.9 1.0 O A:HOH760 3.9 39.2 1.0 OG A:SER30 4.1 12.8 1.0 O A:HOH604 4.2 14.7 1.0 OE2 A:GLU17 4.5 12.8 1.0 O A:HOH703 4.6 47.7 1.0 O A:HOH654 4.6 34.1 1.0 CB A:SER30 4.7 10.2 1.0 O A:HOH730 4.8 27.1 1.0

R.Takeda, E.Tsutsumi, K.Okatsu, S.Fukai, K.Takeda. Structural Characterization of Green Fluorescent Protein in the I-State. Sci Rep V. 14 22832 2024.
ISSN: ESSN 2045-2322
PubMed: 39353998
DOI: 10.1038/S41598-024-73696-Y