Magnesium in PDB 8zus: Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5

The structure of Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5, PDB code: 8zus was solved by R.Takeda, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.02 / 1.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.72, 62.236, 68.343, 90, 90, 90
R / Rfree (%)	13.3 / 16.5

The binding sites of Magnesium atom in the Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5 (pdb code 8zus). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5, PDB code: 8zus:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:9.6 occ:0.25 O A:HOH800 2.0 16.8 0.7 O A:HOH831 2.1 15.4 0.4 O A:HOH816 2.1 19.5 1.0 O A:HOH899 2.1 25.5 0.3 O A:HOH894 2.2 19.5 0.4 O A:HOH800 2.2 12.4 0.3 O A:HOH894 2.2 10.7 0.6 O A:HOH860 2.3 16.7 1.0 O A:HOH899 2.5 34.9 0.7 O A:HOH875 3.3 56.7 0.7 OD2 A:ASP197 3.9 16.1 1.0 O A:HOH764 3.9 28.0 1.0 OD1 A:ASP197 4.4 18.1 1.0 O A:HOH875 4.4 24.9 0.3 CG A:ASP197 4.5 15.4 1.0 NH2 A:ARG80 4.7 22.0 1.0 NH1 A:ARG80 4.8 20.4 1.0 O A:HOH808 4.8 35.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the F99S/M153T/V163A/T203V Variant of Gfp at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:9.2 occ:0.52 O A:HOH649 2.0 15.9 1.0 O A:HOH673 2.0 21.9 1.0 O A:HOH667 2.1 30.6 1.0 O A:HOH589 2.1 19.2 0.3 O A:HOH589 2.1 16.6 0.7 O A:HOH747 2.1 17.3 0.5 O A:HOH650 2.1 17.4 0.4 O A:HOH747 2.1 13.7 0.5 O A:HOH650 2.2 12.4 0.6 O A:HOH556 3.3 11.6 0.3 O A:HOH592 3.9 11.9 0.3 O A:HOH784 3.9 34.7 1.0 O A:HOH698 4.0 30.6 1.0 O A:HOH550 4.2 33.2 1.0 O A:HOH556 4.2 19.6 0.7 OE2 A:GLU5 4.2 9.6 0.3 NZ A:LYS79 4.2 10.8 0.4 NZ A:LYS79 4.3 14.8 0.3 O A:HOH592 4.4 8.7 0.7 O A:HOH523 4.4 21.9 1.0 O A:HOH798 4.4 25.9 1.0 OG1 A:THR38 4.7 11.4 1.0 CE A:LYS79 4.7 27.7 0.3 CE A:LYS79 4.9 24.8 0.4 O A:HOH554 4.9 18.2 1.0 CG2 A:THR38 5.0 10.2 1.0

R.Takeda, E.Tsutsumi, K.Okatsu, S.Fukai, K.Takeda. Structural Characterization of Green Fluorescent Protein in the I-State. Sci Rep V. 14 22832 2024.
ISSN: ESSN 2045-2322
PubMed: 39353998
DOI: 10.1038/S41598-024-73696-Y