Magnesium in PDB 9epn: Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli

All present enzymatic activity of Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli:
2.7.13.3;

The structure of Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli, PDB code: 9epn was solved by A.Koczurowska, G.Bujacz, A.J.Pietrzyk-Brzezinska, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.42 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	64.89, 92.51, 118.16, 90, 90, 90
R / Rfree (%)	18.9 / 21.7

The binding sites of Magnesium atom in the Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli (pdb code 9epn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli, PDB code: 9epn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:36.9 occ:1.00 O2G A:AGS501 2.0 34.9 1.0 OD1 A:ASN359 2.0 36.1 1.0 O2A A:AGS501 2.0 33.5 1.0 O2B A:AGS501 2.1 36.2 1.0 O A:HOH662 2.2 45.9 1.0 O A:HOH678 2.2 40.6 1.0 CG A:ASN359 3.0 36.0 1.0 PB A:AGS501 3.2 37.5 1.0 PG A:AGS501 3.3 42.1 1.0 PA A:AGS501 3.3 35.4 1.0 ND2 A:ASN359 3.4 36.5 1.0 O3A A:AGS501 3.5 37.2 1.0 O3B A:AGS501 3.6 38.7 1.0 O5' A:AGS501 4.1 36.8 1.0 CA A:GLY418 4.2 39.2 1.0 S1G A:AGS501 4.2 50.8 1.0 O A:HOH746 4.3 57.2 1.0 NH2 A:ARG362 4.4 56.0 1.0 CB A:ASN359 4.4 36.8 1.0 O A:ASN355 4.5 39.2 1.0 O3G A:AGS501 4.5 45.1 1.0 N A:GLY418 4.5 38.6 1.0 O1A A:AGS501 4.5 36.3 1.0 CE2 A:TYR363 4.5 38.1 1.0 O1B A:AGS501 4.6 37.7 1.0 OH A:TYR363 4.6 39.9 1.0 CA A:ASN359 4.7 41.0 1.0 N A:ASN359 5.0 37.4 1.0 C A:GLY418 5.0 36.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Hprs Histidine Kinase Cytoplasmic Fragment From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg509 b:38.5 occ:1.00 O2G B:AGS508 2.0 39.9 1.0 O2A B:AGS508 2.1 34.6 1.0 OD1 B:ASN359 2.1 37.2 1.0 O2B B:AGS508 2.1 37.7 1.0 O B:HOH699 2.2 36.7 1.0 O B:HOH698 2.3 41.6 1.0 CG B:ASN359 3.0 36.9 1.0 PB B:AGS508 3.2 38.2 1.0 PA B:AGS508 3.3 37.1 1.0 PG B:AGS508 3.3 39.7 1.0 ND2 B:ASN359 3.3 36.0 1.0 O3A B:AGS508 3.5 37.9 1.0 O3B B:AGS508 3.6 40.2 1.0 O B:HOH732 4.0 51.5 1.0 O5' B:AGS508 4.0 36.7 1.0 CA B:GLY418 4.2 37.0 1.0 NH2 B:ARG362 4.3 60.4 1.0 S1G B:AGS508 4.3 47.9 1.0 CB B:ASN359 4.4 36.6 1.0 O3G B:AGS508 4.5 44.0 1.0 O B:ASN355 4.5 34.9 1.0 O1B B:AGS508 4.5 41.4 1.0 O1A B:AGS508 4.5 33.6 1.0 CE2 B:TYR363 4.5 41.1 1.0 N B:GLY418 4.6 37.1 1.0 OH B:TYR363 4.7 52.2 1.0 CA B:ASN359 4.8 38.1 1.0

A.Koczurowska, D.R.Carrillo, M.G.Alai, M.Zaklos-Szyda, G.Bujacz, A.J.Pietrzyk-Brzezinska. Structural and Biophysical Characterization of the Cytoplasmic Domains of Hprs Kinase and Its Interactions with the Cognate Regulator Hprr. Arch.Biochem.Biophys. V. 764 10269 2024.
ISSN: ESSN 1096-0384
PubMed: 39681306
DOI: 10.1016/J.ABB.2024.110269