Magnesium in PDB 9ff7: Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant

The binding sites of Magnesium atom in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant (pdb code 9ff7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant, PDB code: 9ff7:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1001 b:119.6 occ:1.00 O C:SER346 1.9 111.3 1.0 N C:ILE349 2.0 115.0 1.0 N C:ALA350 2.0 121.9 1.0 C C:ARG347 2.1 114.5 1.0 N C:LYS348 2.4 117.4 1.0 O C:ARG347 2.4 114.5 1.0 C C:SER346 2.5 111.3 1.0 C C:LYS348 2.6 117.4 1.0 CA C:ILE349 2.6 115.0 1.0 C C:ILE349 2.7 115.0 1.0 CA C:ARG347 2.8 114.5 1.0 N C:ARG347 2.9 114.5 1.0 CA C:LYS348 3.0 117.4 1.0 CB C:ILE349 3.2 115.0 1.0 CA C:ALA350 3.2 121.9 1.0 CB C:ALA350 3.4 121.9 1.0 O C:LYS348 3.5 117.4 1.0 OG C:SER346 3.6 111.3 1.0 CA C:SER346 3.8 111.3 1.0 CG1 C:ILE349 3.8 115.0 1.0 CB C:SER346 3.9 111.3 1.0 O C:ILE349 3.9 115.0 1.0 N C:GLU351 4.0 133.1 1.0 CB C:LYS348 4.1 117.4 1.0 C C:ALA350 4.1 121.9 1.0 CB C:ARG347 4.2 114.5 1.0 CB C:ALA357 4.4 118.9 1.0 N C:SER346 4.6 111.3 1.0 CG2 C:ILE349 4.6 115.0 1.0 CD1 C:ILE349 4.9 115.0 1.0 CD C:ARG347 4.9 114.5 1.0 CG C:ARG347 4.9 114.5 1.0

Magnesium binding site 2 out of 4 in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1001 b:124.3 occ:1.00 O D:LEU345 2.1 117.4 1.0 N D:LEU345 2.1 117.4 1.0 C D:HIS344 2.2 118.3 1.0 C D:LEU345 2.3 117.4 1.0 CA D:LEU345 2.6 117.4 1.0 CB D:HIS344 2.7 118.3 1.0 CA D:HIS344 2.8 118.3 1.0 O D:HIS344 2.8 118.3 1.0 N D:SER346 3.3 127.2 1.0 CB D:ASP359 3.7 135.3 1.0 CG D:HIS344 3.8 118.3 1.0 CA D:SER346 4.0 127.2 1.0 CB D:LEU345 4.0 117.4 1.0 CD2 D:HIS344 4.1 118.3 1.0 N D:HIS344 4.2 118.3 1.0 N D:ASP359 4.2 135.3 1.0 O D:VAL177 4.2 104.8 1.0 CB D:SER346 4.5 127.2 1.0 CA D:ASP359 4.6 135.3 1.0 CG D:ASP359 4.8 135.3 1.0 OD2 D:ASP359 4.8 135.3 1.0 CG D:LEU345 4.9 117.4 1.0 CD D:PRO179 4.9 98.0 1.0

Magnesium binding site 3 out of 4 in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg1001 b:85.9 occ:1.00 O I:PRO180 2.0 78.8 1.0 O I:PRO179 2.0 75.4 1.0 N I:ALA182 2.1 75.2 1.0 C I:PRO180 2.2 78.8 1.0 CB I:ALA182 2.5 75.2 1.0 C I:LYS181 2.6 81.6 1.0 C I:PRO179 2.6 75.4 1.0 CA I:ALA182 2.7 75.2 1.0 N I:LYS181 2.7 81.6 1.0 CA I:PRO180 3.0 78.8 1.0 N I:PRO180 3.1 78.8 1.0 CA I:LYS181 3.2 81.6 1.0 O I:LYS181 3.3 81.6 1.0 CA I:PRO179 3.7 75.4 1.0 CG I:PRO179 3.7 75.4 1.0 CB I:PRO179 3.8 75.4 1.0 C I:ALA182 4.1 75.2 1.0 CD I:ARG347 4.1 91.8 1.0 CB I:LYS181 4.2 81.6 1.0 N I:PRO179 4.2 75.4 1.0 CD I:PRO180 4.3 78.8 1.0 CD I:PRO179 4.3 75.4 1.0 CB I:PRO180 4.4 78.8 1.0 N I:GLY183 4.7 78.6 1.0 CG I:PRO180 4.7 78.8 1.0 CB I:ARG347 4.8 91.8 1.0 NH1 I:ARG347 4.8 91.8 1.0 O I:ALA182 4.9 75.2 1.0 C I:ALA178 5.0 72.6 1.0 CA I:ARG347 5.0 91.8 1.0

Magnesium binding site 4 out of 4 in the Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of the Bmoe-Crosslinked Transcription Termination Factor Rho in the Presence of Ppgpp; S84C/M405C Double Mutant within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1001 b:89.3 occ:1.00 O J:HIS344 2.1 83.0 1.0 CA J:LEU345 2.4 80.0 1.0 CA J:ILE358 2.5 83.3 1.0 O J:ALA357 2.5 88.0 1.0 CD2 J:LEU345 2.6 80.0 1.0 C J:HIS344 2.7 83.0 1.0 N J:LEU345 2.8 80.0 1.0 N J:ASP359 3.0 89.3 1.0 C J:ILE358 3.2 83.3 1.0 CB J:LEU345 3.2 80.0 1.0 C J:ALA357 3.3 88.0 1.0 N J:ILE358 3.3 83.3 1.0 CB J:ILE358 3.3 83.3 1.0 CG J:LEU345 3.4 80.0 1.0 CG1 J:ILE358 3.4 83.3 1.0 C J:LEU345 3.4 80.0 1.0 N J:SER346 3.7 86.7 1.0 CG2 J:ILE358 3.7 83.3 1.0 CA J:HIS344 4.1 83.0 1.0 CA J:ASP359 4.3 89.3 1.0 O J:ILE358 4.4 83.3 1.0 N J:HIS344 4.4 83.0 1.0 O J:LEU345 4.5 80.0 1.0 CB J:ASP359 4.6 89.3 1.0 CD1 J:ILE358 4.7 83.3 1.0 CD1 J:LEU345 4.7 80.0 1.0 CA J:ALA357 4.8 88.0 1.0 CG J:LEU343 4.9 81.5 1.0 C J:LEU343 5.0 81.5 1.0 CB J:HIS344 5.0 83.0 1.0 CA J:SER346 5.0 86.7 1.0

B.Wang, N.Said, T.Hilal, M.Finazzo, M.C.Wahl, I.Artsimovitch. Nucleotide-Induced Hyper-Oligomerization Inactivates Transcription Termination Factor Rho. Nat Commun V. 16 1653 2025.
ISSN: ESSN 2041-1723
PubMed: 39952913
DOI: 10.1038/S41467-025-56824-8