Magnesium in PDB 9fnn: Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli

All present enzymatic activity of Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli:
2.4.1.12;

The binding sites of Magnesium atom in the Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli (pdb code 9fnn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli, PDB code: 9fnn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ Q:Mg302 b:44.6 occ:1.00 OG1 Q:THR16 2.1 49.2 1.0 O3B Q:ATP301 2.7 20.0 1.0 O2G Q:ATP301 3.1 20.0 1.0 CB Q:THR16 3.3 42.3 1.0 PG Q:ATP301 3.5 20.0 1.0 O2B Q:ATP301 3.6 20.0 1.0 PB Q:ATP301 3.7 20.0 1.0 OD2 Q:ASP124 3.7 62.4 1.0 O3A Q:ATP301 4.0 20.0 1.0 N Q:THR16 4.1 44.2 1.0 CG2 Q:THR16 4.2 46.4 1.0 CA Q:THR16 4.2 39.5 1.0 O1G Q:ATP301 4.5 20.0 1.0 SG Q:CYS39 4.5 73.0 0.8 O3G Q:ATP301 4.5 20.0 1.0 CD1 Q:LEU43 4.5 52.6 1.0 CG Q:ASP124 4.6 58.6 1.0 OD1 Q:ASP124 4.6 57.8 1.0 CB Q:CYS39 4.8 54.9 0.8 CB Q:LEU43 4.9 45.9 1.0 O1B Q:ATP301 5.0 20.0 1.0 PA Q:ATP301 5.0 20.0 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of the C-Di-Gmp-Saturated 'Crown'Less Bcs Macrocomplex For Cellulose Secretion in E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ W:Mg302 b:30.0 occ:1.00 O3G W:ATP301 2.1 20.0 1.0 O2B W:ATP301 2.1 20.0 1.0 OG1 W:THR16 2.2 49.4 1.0 PB W:ATP301 3.3 20.0 1.0 PG W:ATP301 3.4 20.0 1.0 CB W:THR16 3.4 40.2 1.0 O3B W:ATP301 3.6 20.0 1.0 N W:THR16 4.0 37.0 1.0 OD2 W:ASP124 4.0 56.6 1.0 O1B W:ATP301 4.1 20.0 1.0 CA W:THR16 4.2 35.7 1.0 OD2 W:ASP41 4.3 60.0 1.0 O1G W:ATP301 4.3 20.0 1.0 O2G W:ATP301 4.4 20.0 1.0 OD1 W:ASP124 4.4 56.8 1.0 CG2 W:THR16 4.5 42.6 1.0 O3A W:ATP301 4.5 20.0 1.0 CG W:ASP124 4.6 55.9 1.0 SG W:CYS39 4.7 59.1 0.7 O1A W:ATP301 4.7 20.0 1.0 CB W:THR15 4.7 40.9 1.0 C W:THR15 4.9 37.2 1.0 CB W:CYS39 5.0 46.0 0.7 OD1 W:ASP41 5.0 57.8 1.0

I.Anso, S.Zouhir, T.G.Sana, P.V.Krasteva. Structural Basis For Synthase Activation and Cellulose Modification in the E. Coli Type II Bcs Secretion System. Nat Commun 2024.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-024-53113-8