Magnesium in PDB 9fyx: Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex

All present enzymatic activity of Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex:
2.7.7.48; 2.7.7.6;

The structure of Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex also contains other interesting chemical elements:

Zinc

(Zn)

9 atoms

The binding sites of Magnesium atom in the Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex (pdb code 9fyx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex, PDB code: 9fyx:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg4003 b:32.4 occ:1.00 OD1 A:ASP497 2.0 26.1 1.0 OD2 A:ASP495 2.1 35.0 1.0 OD1 A:ASP495 2.1 31.4 1.0 OD2 A:ASP497 2.2 33.5 1.0 CG A:ASP497 2.4 29.4 1.0 CG A:ASP495 2.4 35.5 1.0 OD1 A:ASP499 3.2 34.7 1.0 O3' P:C35 3.7 26.1 1.0 CB A:ASP495 3.9 24.6 1.0 CB A:ASP497 3.9 19.3 1.0 CG A:ASP499 3.9 32.7 1.0 OD2 A:ASP499 4.0 34.5 1.0 NZ B:LYS942 4.3 31.4 1.0 O A:ASP495 4.4 27.0 1.0 O A:ASP497 4.5 28.7 1.0 N A:ASP497 4.6 19.9 1.0 C5' P:C35 4.6 17.1 1.0 CA A:ASP497 4.6 11.8 1.0 CA A:ASP495 4.7 19.4 1.0 C A:ASP495 4.7 25.8 1.0 N A:ASP495 4.7 22.6 1.0 C3' P:C35 4.7 21.4 1.0 C A:ASP497 4.7 22.7 1.0 OP1 P:C35 4.9 29.4 1.0

Magnesium binding site 2 out of 2 in the Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Influenza A/H7N9 Polymerase Pre-Cleavage Cap-Snatching Complex within 5.0Å range: probe atom residue distance (Å) B Occ a:Mg1001 b:42.9 occ:1.00 OD2 a:ASP119 2.2 100.9 1.0 OP1 P:A12 2.2 111.9 1.0 OD2 a:ASP108 2.6 97.8 1.0 CG a:ASP119 3.0 99.7 1.0 OD1 a:ASP119 3.2 99.0 1.0 OD1 a:ASP108 3.3 89.2 1.0 CG a:ASP108 3.3 93.8 1.0 O a:ILE120 3.3 97.3 1.0 P P:A12 3.5 115.5 1.0 C5' P:A12 3.5 111.9 1.0 CE1 a:HIS41 3.7 97.6 1.0 OE2 a:GLU80 3.8 94.0 1.0 O5' P:A12 3.8 113.0 1.0 NZ a:LYS134 4.0 67.4 1.0 OP2 P:A13 4.0 100.4 1.0 NE2 a:HIS41 4.1 99.2 1.0 C a:ILE120 4.2 97.2 1.0 N a:ILE120 4.3 99.2 1.0 CB a:ASP119 4.4 95.8 1.0 O3' P:G11 4.5 117.7 1.0 OP2 P:A12 4.5 116.6 1.0 ND1 a:HIS41 4.7 98.4 1.0 C4' P:A12 4.7 113.2 1.0 CB a:ASP108 4.7 91.2 1.0 C a:ASP119 4.7 94.9 1.0 CA a:ASP119 4.7 93.5 1.0 CD a:GLU80 4.8 94.9 1.0 CE a:LYS134 4.8 65.8 1.0 O3' P:A12 4.9 112.3 1.0 CA a:ILE120 4.9 99.4 1.0

A.H.Rotsch, D.Li, M.Dupont, T.Krischuns, C.Oberthuer, A.Stelfox, M.Lukarska, I.Fianu, M.Lidschreiber, N.Naffakh, C.Dienemann, S.Cusack, P.Cramer. Mechanism of Co-Transcriptional Cap-Snatching By Influenza Polymerase To Be Published.