Magnesium in PDB 9kor: Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex

The binding sites of Magnesium atom in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex (pdb code 9kor). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex, PDB code: 9kor:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1501 b:15.6 occ:1.00 OP2 B:A72 2.9 18.3 1.0 CE2 A:PHE66 3.5 18.2 1.0 NE A:ARG98 3.7 20.2 1.0 NH2 A:ARG98 3.8 14.3 1.0 CZ A:ARG98 3.9 18.1 1.0 OP2 B:A73 3.9 26.3 1.0 CD2 A:PHE66 3.9 21.0 1.0 P B:A72 4.0 15.5 1.0 O5' B:A72 4.2 22.4 1.0 CZ A:PHE66 4.3 15.3 1.0 N7 B:A73 4.4 17.0 1.0 O4 B:U74 4.4 27.9 1.0 N7 B:A72 4.4 15.4 1.0 CD A:ARG98 4.5 14.1 1.0 C8 B:A72 4.5 15.3 1.0 OP1 B:A72 4.5 21.2 1.0 NH1 A:ARG98 4.7 13.0 1.0

Magnesium binding site 2 out of 4 in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:61.5 occ:1.00 O4 B:U26 4.3 17.6 1.0 O4 B:U25 4.5 23.8 1.0 O4 B:U69 4.6 17.9 1.0 O6 B:G70 4.7 22.9 1.0 N3 B:U25 4.8 19.7 1.0

Magnesium binding site 3 out of 4 in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:20.5 occ:1.00 OP1 B:A114 3.7 44.4 1.0 OP2 B:A114 3.8 30.8 1.0 C5' B:A114 3.8 42.6 1.0 P B:A114 4.1 37.5 1.0 OP2 B:C97 4.2 32.0 1.0 OP2 B:U115 4.3 39.1 1.0 O6 B:G99 4.3 35.7 1.0 O5' B:A114 4.4 42.1 1.0 NH2 A:ARG279 4.5 19.6 1.0 C5 B:C97 4.5 20.4 1.0 N7 B:G98 4.6 28.4 1.0 O6 B:G98 4.7 23.6 1.0 N4 B:C116 4.8 16.7 1.0 N4 B:C97 4.9 19.2 1.0 C4 B:C97 5.0 21.6 1.0

Magnesium binding site 4 out of 4 in the Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of PSCAS9-Sgrna-Dsdna Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg203 b:43.1 occ:1.00 N7 B:G105 2.6 22.1 1.0 C8 B:G105 3.2 21.3 1.0 OP2 B:G105 3.4 31.5 1.0 C5 B:G105 3.7 22.0 1.0 N9 B:G105 4.3 19.8 1.0 O6 B:G105 4.4 21.2 1.0 C6 B:G105 4.5 23.3 1.0 O5' B:G105 4.5 33.2 1.0 P B:G105 4.6 33.8 1.0 C4 B:G105 4.6 19.8 1.0 C4 B:U104 4.7 25.4 1.0 C5 B:U104 4.7 27.2 1.0 N3 B:U104 4.8 19.6 1.0 C6 B:U104 4.8 25.2 1.0 C3' B:U104 4.9 34.6 1.0 C2 B:U104 4.9 21.5 1.0 N1 B:U104 4.9 21.5 1.0

P.Shen, X.Lu, C.Wang, J.W.Huang, J.Min, J.Luo, Y.Sun, C.C.Chen, R.T.Guo. Structural and Mechanistic Investigations of A High-Fidelity CAS9 From Parasutterella Secunda Acs Catalysis V. 15 10109 2025.
ISSN: ESSN 2155-5435
DOI: 10.1021/ACSCATAL.5C02993