Magnesium in PDB 1nke: A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp.

Enzymatic activity of A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp.

All present enzymatic activity of A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp.:
2.7.7.7;

Protein crystallography data

The structure of A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp., PDB code: 1nke was solved by S.J.Johnson, L.S.Beese, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	43.07 / 1.80
*Space group*	P 2₁ 2₁ 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	88.294, 94.322, 105.685, 90.00, 90.00, 90.00
*R / R_free (%)*	21.6 / 24.8

Magnesium Binding Sites:

The binding sites of Magnesium atom in the A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp. (pdb code 1nke). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp., PDB code: 1nke:

Magnesium binding site 1 out of 1 in 1nke

Go back to

Magnesium Binding Sites List in 1nke

Magnesium binding site 1 out of 1 in the A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp.

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of A Bacillus Dna Polymerase I Product Complex Bound to A Cytosine-Thymine Mismatch After A Single Round of Primer Extension, Following Incorporation of Dctp. within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg920 b:38.6 occ:1.00	OD1	A:ASP653	2.8	22.8	1.0
	O	A:TYR654	2.8	17.3	1.0
	OD2	A:ASP830	2.9	19.5	0.5
	O	A:HOH2525	3.0	26.2	1.0
	CG	A:ASP653	3.7	21.4	1.0
	CG	A:ASP830	3.9	19.7	0.5
	O	A:HOH2512	3.9	19.6	1.0
	CB	A:ASP653	4.0	19.3	1.0
	C	A:TYR654	4.0	16.8	1.0
	O	A:HOH2845	4.1	36.2	1.0
	OD1	A:ASP830	4.2	19.1	0.5
	O	A:HOH2645	4.4	38.8	1.0
	O	A:HOH3289	4.4	33.7	1.0
	CA	A:SER655	4.6	17.2	1.0
	N	A:TYR654	4.7	16.9	1.0
	N	A:SER655	4.7	16.8	1.0
	C	A:ASP653	4.8	16.9	1.0
	OD2	A:ASP653	4.8	22.0	1.0
	N	A:GLN656	4.9	16.2	1.0

Reference:

S.J.Johnson, L.S.Beese. Structures of Mismatch Replication Errors Observed in A Dna Polymerase. Cell(Cambridge,Mass.) V. 116 803 2004.
ISSN: ISSN 0092-8674
PubMed: 15035983
DOI: 10.1016/S0092-8674(04)00252-1

Page generated: Sun Aug 10 01:46:45 2025

Last articles

Mg in 3TTZ
Mg in 3TVK
Mg in 3TVB
Mg in 3TVA
Mg in 3TUY
Mg in 3TTE
Mg in 3TTF
Mg in 3TTD
Mg in 3TTC
Mg in 3TSU

	© Copyright 2008-2020 by atomistry.com
Home \| Site Map \| Copyright \| Contact us \| Privacy