Magnesium in PDB 1qyf: Crystal Structure of Matured Green Fluorescent Protein R96A Variant

The structure of Crystal Structure of Matured Green Fluorescent Protein R96A Variant, PDB code: 1qyf was solved by D.P.Barondeau, C.D.Putnam, C.J.Kassmann, J.A.Tainer, E.D.Getzoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.515, 62.388, 71.038, 90.00, 90.00, 90.00
R / Rfree (%)	14.4 / 21.4

The binding sites of Magnesium atom in the Crystal Structure of Matured Green Fluorescent Protein R96A Variant (pdb code 1qyf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Matured Green Fluorescent Protein R96A Variant, PDB code: 1qyf:

Magnesium binding site 1 out of 1 in the Crystal Structure of Matured Green Fluorescent Protein R96A Variant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Matured Green Fluorescent Protein R96A Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:19.0 occ:1.00 O2 A:EDO303 2.0 17.7 1.0 O A:HOH1285 2.0 18.6 1.0 O A:HOH1288 2.0 18.5 1.0 O A:HOH1289 2.1 21.3 1.0 O A:HOH1286 2.1 18.7 1.0 O A:HOH1287 2.1 15.2 1.0 C2 A:EDO303 2.9 21.4 1.0 O1 A:EDO303 3.8 19.2 1.0 OE2 A:GLU142 3.9 16.9 1.0 C1 A:EDO303 3.9 23.4 1.0 OE1 A:GLU142 4.1 17.4 1.0 O A:HOH1140 4.2 38.4 1.0 O A:HOH1164 4.3 29.6 1.0 CA A:GLY174 4.3 17.9 1.0 CD A:GLU142 4.4 15.8 1.0 O A:HOH1165 4.5 42.7 1.0 N A:GLY174 4.9 20.1 1.0 O A:ILE171 5.0 16.8 1.0

D.P.Barondeau, C.D.Putnam, C.J.Kassmann, J.A.Tainer, E.D.Getzoff. Mechanism and Energetics of Green Fluorescent Protein Chromophore Synthesis Revealed By Trapped Intermediate Structures. Proc.Natl.Acad.Sci.Usa V. 100 12111 2003.
ISSN: ISSN 0027-8424
PubMed: 14523232
DOI: 10.1073/PNAS.2133463100