Magnesium in PDB 1rkv: Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa

The structure of Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa, PDB code: 1rkv was solved by S.K.Singh, K.Yang, K.Subramanian, S.Karthikeyan, T.Huynh, X.Zhang, M.A.Phillips, H.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.88 / 1.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	71.751, 93.021, 132.348, 90.00, 90.00, 90.00
R / Rfree (%)	18.7 / 23

The binding sites of Magnesium atom in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa (pdb code 1rkv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa, PDB code: 1rkv:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:25.2 occ:1.00 OD2 A:ASP7 2.1 24.0 1.0 O A:GLU9 2.1 20.9 1.0 O2 A:PO4320 2.2 36.1 1.0 O A:HOH526 2.2 23.4 1.0 OD1 A:ASP152 2.2 23.9 1.0 O A:HOH538 2.2 23.5 1.0 CG A:ASP152 3.2 25.1 1.0 CG A:ASP7 3.2 25.1 1.0 C A:GLU9 3.3 23.0 1.0 OD2 A:ASP152 3.4 24.1 1.0 P A:PO4320 3.5 44.5 1.0 OD1 A:ASP7 3.7 26.7 1.0 O A:HOH546 3.9 29.5 1.0 OG A:SER153 4.0 33.5 1.0 OD2 A:ASP156 4.0 25.7 1.0 O3 A:PO4320 4.0 38.5 1.0 O1 A:PO4320 4.0 36.9 1.0 CA A:GLU9 4.1 22.3 1.0 N A:GLU9 4.1 19.8 1.0 CB A:GLU9 4.2 21.9 1.0 OE2 A:GLU15 4.3 31.8 1.0 N A:GLY10 4.3 24.4 1.0 O A:HOH722 4.3 43.8 1.0 CB A:ASP7 4.4 19.8 1.0 CA A:GLY10 4.5 25.1 1.0 CB A:ASP152 4.5 23.9 1.0 OD1 A:ASN155 4.6 32.6 1.0 O4 A:PO4320 4.6 42.3 1.0 N A:ASP152 4.7 21.7 1.0 OD1 A:ASP156 4.8 23.9 1.0 CG A:ASP156 4.8 24.5 1.0 CB A:SER153 4.8 25.0 1.0 C A:LEU8 4.9 20.5 1.0 N A:SER153 5.0 24.5 1.0

Magnesium binding site 2 out of 4 in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:37.2 occ:1.00 O A:HOH649 2.2 34.1 1.0 OD2 A:ASP152 2.2 24.1 1.0 O A:HOH789 2.3 39.4 1.0 O A:HOH572 2.3 29.3 1.0 O A:HOH717 2.4 41.7 1.0 O A:HOH794 2.5 39.0 1.0 CG A:ASP152 3.3 25.1 1.0 CB A:ASP152 3.6 23.9 1.0 O A:HIS170 4.0 27.4 1.0 O A:HOH538 4.0 23.5 1.0 O A:ASP152 4.1 25.1 1.0 CA A:GLY10 4.2 25.1 1.0 O A:HOH770 4.2 61.0 1.0 OG A:SER153 4.3 33.5 1.0 OE2 A:GLU15 4.3 31.8 1.0 OD1 A:ASP152 4.4 23.9 1.0 O A:HOH785 4.5 64.3 1.0 C A:ASP152 4.6 25.0 1.0 O A:GLY10 4.6 25.7 1.0 O A:HOH759 4.7 57.0 1.0 O A:HOH657 4.7 37.6 1.0 CA A:ASP152 4.8 24.6 1.0 C A:GLY10 4.8 26.2 1.0 C A:HIS170 5.0 27.6 1.0

Magnesium binding site 3 out of 4 in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg403 b:29.5 occ:1.00 OD2 B:ASP7 2.1 29.1 1.0 O B:GLU9 2.1 23.6 1.0 OD1 B:ASP152 2.1 26.7 1.0 O B:HOH542 2.2 25.1 1.0 O B:HOH552 2.3 27.4 1.0 O2 B:PO4321 2.3 32.6 1.0 CG B:ASP7 3.1 26.4 1.0 CG B:ASP152 3.1 28.3 1.0 C B:GLU9 3.2 24.3 1.0 OD2 B:ASP152 3.4 30.9 1.0 OD1 B:ASP7 3.5 28.4 1.0 P B:PO4321 3.8 43.2 1.0 CA B:GLU9 3.9 23.0 1.0 OD2 B:ASP156 4.0 29.3 1.0 N B:GLU9 4.1 21.2 1.0 CB B:GLU9 4.1 23.7 1.0 O B:HOH701 4.2 43.1 1.0 N B:GLY10 4.2 22.4 1.0 OG B:SER153 4.3 39.1 1.0 OE2 B:GLU15 4.3 45.8 1.0 O1 B:PO4321 4.3 31.7 1.0 O3 B:PO4321 4.4 34.7 1.0 CB B:ASP7 4.4 23.9 1.0 CA B:GLY10 4.4 25.5 1.0 CB B:ASP152 4.4 26.4 1.0 N B:ASP152 4.7 24.0 1.0 O4 B:PO4321 4.8 40.6 1.0 OD1 B:ASN155 4.9 46.4 1.0 C B:LEU8 4.9 21.4 1.0 N B:VAL11 4.9 26.8 1.0 CG B:ASP156 4.9 28.2 1.0 CG2 B:VAL11 4.9 22.1 1.0 OD1 B:ASP156 4.9 28.3 1.0

Magnesium binding site 4 out of 4 in the Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of Phosphate Complex of Thrh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg404 b:55.5 occ:1.00 OD2 B:ASP152 2.5 30.9 1.0 O B:HIS170 3.1 31.7 1.0 CG B:ASP152 3.4 28.3 1.0 CB B:ASP152 3.6 26.4 1.0 CA B:GLY10 4.0 25.5 1.0 C B:HIS170 4.2 31.0 1.0 CB B:HIS170 4.2 32.9 1.0 O B:ASP152 4.3 29.5 1.0 O B:GLY10 4.4 29.2 1.0 OD1 B:ASP152 4.6 26.7 1.0 C B:GLY10 4.6 26.0 1.0 O B:HOH552 4.8 27.4 1.0 CA B:ASP152 4.9 26.6 1.0 C B:ASP152 4.9 28.6 1.0 OE2 B:GLU15 4.9 45.8 1.0 CA B:HIS170 4.9 31.0 1.0

S.K.Singh, K.Yang, S.Karthikeyan, T.Huynh, X.Zhang, M.A.Phillips, H.Zhang. The Thrh Gene Product of Pseudomonas Aeruginosa Is A Dual Activity Enzyme with A Novel Phosphoserine:Homoserine Phosphotransferase Activity. J.Biol.Chem. V. 279 13166 2004.
ISSN: ISSN 0021-9258
PubMed: 14699121
DOI: 10.1074/JBC.M311393200