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Magnesium in PDB 1sks: Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template

Enzymatic activity of Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template

All present enzymatic activity of Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template:
2.7.7.7;

Protein crystallography data

The structure of Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template, PDB code: 1sks was solved by Y.Li, S.Dutta, S.Doublie, H.M.Bdour, J.S.Taylor, T.Ellenberger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.30
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 104.773, 214.917, 52.257, 90.00, 90.00, 90.00
R / Rfree (%) 23.5 / 26.6

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template (pdb code 1sks). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template, PDB code: 1sks:

Magnesium binding site 1 out of 1 in 1sks

Go back to Magnesium Binding Sites List in 1sks
Magnesium binding site 1 out of 1 in the Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Binary 3' Complex of T7 Dna Polymerase with A Dna Primer/Template Containing A Cis-Syn Thymine Dimer on the Template within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg4003

b:68.5
occ:1.00
 O A:HOH5257 2.7 58.7 1.0
O A:HOH5255 2.7 60.1 1.0
O A:HOH5256 2.8 54.7 1.0
O A:HOH5012 2.8 37.1 1.0
OD1 A:ASP5 3.6 34.6 1.0
O A:HOH5149 4.2 53.1 1.0
O A:ILE6 4.4 28.5 1.0
CG A:ASP5 4.5 32.7 1.0
OD2 A:ASP5 4.6 38.1 1.0
CE A:MET107 4.6 49.8 1.0
OD1 A:ASP65 4.9 33.8 1.0
OD2 A:ASP65 4.9 31.5 1.0

Reference:

Y.Li, S.Dutta, S.Doublie, H.M.Bdour, J.S.Taylor, T.Ellenberger. Nucleotide Insertion Opposite A Cis-Syn Thymine Dimer By A Replicative Dna Polymerase From Bacteriophage T7. Nat.Struct.Mol.Biol. V. 11 784 2004.
ISSN: ISSN 1545-9993
PubMed: 15235589
DOI: 10.1038/NSMB792
Page generated: Tue Aug 13 13:45:38 2024

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