Magnesium in PDB 2a0i: F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna

The structure of F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna, PDB code: 2a0i was solved by C.Larkin, S.Datta, M.J.Harley, B.J.Anderson, A.Ebie, V.Hargreaves, J.F.Schildbach, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.87 / 2.72
Space group	P 62
Cell size a, b, c (Å), α, β, γ (°)	77.515, 77.515, 114.369, 90.00, 90.00, 120.00
R / Rfree (%)	21.5 / 27.2

The binding sites of Magnesium atom in the F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna (pdb code 2a0i). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna, PDB code: 2a0i:

Magnesium binding site 1 out of 1 in the F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of F Factor Trai Relaxase Domain Bound to F Orit Single-Stranded Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg900 b:52.4 occ:1.00 OP1 B:DG9 2.1 63.8 1.0 ND1 A:HIS146 2.2 71.9 1.0 NE2 A:HIS159 2.4 46.6 1.0 NE2 A:HIS157 2.5 58.9 1.0 O3' B:DT8 2.6 57.6 1.0 P B:DG9 2.9 59.7 1.0 CE1 A:HIS146 2.9 73.4 1.0 CD2 A:HIS159 3.1 47.7 1.0 CE1 A:HIS157 3.3 59.2 1.0 CG A:HIS146 3.3 68.4 1.0 CD2 A:HIS157 3.4 58.6 1.0 CE1 A:HIS159 3.5 47.8 1.0 CB A:HIS146 3.9 63.9 1.0 C3' B:DT8 3.9 53.8 1.0 OP2 B:DG9 4.0 63.1 1.0 O5' B:DG9 4.0 62.6 1.0 NE2 A:HIS146 4.1 73.2 1.0 C5' B:DG9 4.2 67.5 1.0 CD2 A:HIS146 4.3 70.6 1.0 CG A:HIS159 4.3 48.4 1.0 NZ A:LYS265 4.4 94.9 1.0 ND1 A:HIS157 4.4 58.0 1.0 C4' B:DT8 4.4 53.0 1.0 ND1 A:HIS159 4.5 48.5 1.0 CG A:HIS157 4.5 56.9 1.0 C2' B:DT8 4.7 52.8 1.0 O A:HOH956 4.9 46.6 1.0 CE A:LYS265 5.0 94.5 1.0

C.Larkin, S.Datta, M.J.Harley, B.J.Anderson, A.Ebie, V.Hargreaves, J.F.Schildbach. Inter- and Intramolecular Determinants of the Specificity of Single-Stranded Dna Binding and Cleavage By the F Factor Relaxase. Structure V. 13 1533 2005.
ISSN: ISSN 0969-2126
PubMed: 16216584
DOI: 10.1016/J.STR.2005.06.013