Magnesium in PDB 2c77: Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A

All present enzymatic activity of Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A:
3.1.5.1;

The structure of Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A, PDB code: 2c77 was solved by A.Parmeggiani, I.M.Krab, S.Okamura, R.C.Nielsen, J.Nyborg, P.Nissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.70 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	43.320, 94.580, 104.380, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 21.8

The binding sites of Magnesium atom in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A (pdb code 2c77). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A, PDB code: 2c77:

Magnesium binding site 1 out of 1 in the Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ef-Tu Complexed with A Gtp Analog and the Antibiotic GE2270 A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1407 b:9.0 occ:1.00 O1G A:GNP1406 2.0 9.7 1.0 OG1 A:THR25 2.1 9.4 1.0 O2B A:GNP1406 2.1 8.1 1.0 OG1 A:THR62 2.1 11.2 1.0 O A:HOH2082 2.1 7.4 1.0 O A:HOH2042 2.1 9.9 1.0 CB A:THR62 3.1 13.3 1.0 CB A:THR25 3.2 9.3 1.0 PG A:GNP1406 3.2 8.9 1.0 PB A:GNP1406 3.2 9.4 1.0 N3B A:GNP1406 3.3 9.4 1.0 N A:THR25 3.7 6.8 1.0 O3G A:GNP1406 4.0 10.2 1.0 CA A:THR25 4.1 8.2 1.0 OD1 A:ASP81 4.1 10.8 1.0 OD2 A:ASP51 4.1 13.9 1.0 N A:THR62 4.1 13.3 1.0 CA A:THR62 4.1 13.3 1.0 CG2 A:THR62 4.1 15.1 1.0 OD1 A:ASP51 4.2 15.8 1.0 O2A A:GNP1406 4.2 10.9 1.0 O1B A:GNP1406 4.2 8.0 1.0 O2G A:GNP1406 4.3 9.0 1.0 CG2 A:THR25 4.3 11.2 1.0 O3A A:GNP1406 4.3 9.1 1.0 OD2 A:ASP81 4.4 8.0 1.0 CB A:LYS24 4.5 5.8 1.0 O A:CYS82 4.5 8.2 1.0 CG A:ASP51 4.6 16.3 1.0 CE A:LYS24 4.6 6.6 1.0 CG A:ASP81 4.6 10.3 1.0 PA A:GNP1406 4.7 9.6 1.0 C A:LYS24 4.7 6.9 1.0 O1A A:GNP1406 4.8 8.6 1.0

A.Parmeggiani, I.M.Krab, S.Okamura, R.C.Nielsen, J.Nyborg, P.Nissen. Structural Basis of the Action of Pulvomycin and GE2270 A on Elongation Factor Tu. Biochemistry V. 45 6846 2006.
ISSN: ISSN 0006-2960
PubMed: 16734421
DOI: 10.1021/BI0525122