Magnesium in PDB 2cl0: Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp

The structure of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp, PDB code: 2cl0 was solved by B.U.Klink, R.S.Goody, A.J.Scheidig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.42 / 1.8
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.540, 88.540, 144.100, 90.00, 90.00, 120.00
R / Rfree (%)	14.6 / 18.5

The binding sites of Magnesium atom in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp (pdb code 2cl0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp, PDB code: 2cl0:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg167 b:17.3 occ:0.33 O X:HOH2110 1.9 20.5 1.0 O X:HOH2119 1.9 21.4 1.0 O X:THR124 3.8 22.8 1.0 OE1 X:GLN129 3.9 31.8 1.0 O X:HOH2111 4.1 28.3 1.0 NE2 X:GLN129 4.2 21.0 1.0 O X:HOH2116 4.2 55.3 1.0 CD X:GLN129 4.5 26.9 1.0 C X:THR124 4.9 23.4 1.0

Magnesium binding site 2 out of 5 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg168 b:22.3 occ:1.00 O1G X:GNP200 1.9 24.7 1.0 O X:HOH2067 2.0 27.4 1.0 O2B X:GNP200 2.0 23.3 1.0 OG X:SER17 2.0 23.4 1.0 O X:HOH2034 2.1 24.9 1.0 O X:HOH2161 2.1 27.6 1.0 CB X:SER17 3.2 24.9 1.0 PB X:GNP200 3.2 24.5 1.0 PG X:GNP200 3.2 26.9 1.0 N3B X:GNP200 3.5 22.9 1.0 O X:HOH2035 3.9 30.7 1.0 N X:SER17 3.9 24.9 1.0 OD2 X:ASP57 4.1 31.9 1.0 CA X:SER17 4.1 24.3 1.0 O2A X:GNP200 4.2 28.7 1.0 O1B X:GNP200 4.2 22.7 1.0 O X:ASP33 4.2 46.9 0.7 OD1 X:ASP57 4.2 27.8 1.0 O X:ASP33 4.3 47.0 0.3 O X:HOH2033 4.3 39.9 1.0 O3G X:GNP200 4.3 25.6 1.0 O2G X:GNP200 4.3 25.8 1.0 O3A X:GNP200 4.3 23.7 1.0 CB X:ASP33 4.5 47.2 0.3 O X:THR35 4.5 46.0 1.0 O X:THR58 4.5 27.0 1.0 CG X:ASP57 4.6 25.8 1.0 PA X:GNP200 4.6 26.1 1.0 CB X:ASP33 4.6 47.0 0.7 O1A X:GNP200 4.7 25.9 1.0 CB X:LYS16 4.8 25.7 1.0 O X:HOH2030 4.8 38.0 1.0 CG X:ASP33 4.8 47.4 0.3 CE X:LYS16 4.9 26.5 1.0 C X:LYS16 4.9 24.5 1.0

Magnesium binding site 3 out of 5 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg169 b:37.9 occ:1.00 O X:HOH2113 2.1 35.8 1.0 O X:HOH2112 2.1 43.8 1.0 O X:HOH2106 4.1 32.0 1.0 OE1 X:GLU126 4.2 33.9 1.0 OE2 X:GLU126 4.2 31.0 1.0 O X:HOH2109 4.3 26.9 1.0 CD X:GLU126 4.7 31.5 1.0 CD X:ARG123 5.0 26.0 1.0

Magnesium binding site 4 out of 5 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg170 b:28.8 occ:1.00 O3G X:GNP200 2.0 25.6 1.0 O X:HOH2030 2.1 38.0 1.0 O X:HOH2035 2.1 30.7 1.0 O X:HOH2072 2.1 31.1 1.0 OE1 X:GLU63 2.1 36.6 1.0 O X:HOH2074 2.1 28.3 1.0 CD X:GLU63 3.1 46.3 1.0 PG X:GNP200 3.3 26.9 1.0 OE2 X:GLU63 3.4 42.6 1.0 O1G X:GNP200 3.7 24.7 1.0 O X:HOH2165 3.8 47.4 1.0 O X:HOH2073 4.0 34.5 1.0 O X:ASP33 4.0 47.0 0.3 O X:ASP33 4.0 46.9 0.7 O X:HOH2166 4.1 36.0 1.0 O2G X:GNP200 4.3 25.8 1.0 N3B X:GNP200 4.3 22.9 1.0 O X:THR35 4.3 46.0 1.0 CG X:GLU63 4.4 46.5 1.0 O X:PRO34 4.6 45.4 1.0 O X:HOH2161 4.6 27.6 1.0 C X:PRO34 4.8 46.2 1.0 CB X:ALA59 4.9 29.0 1.0 CA X:PRO34 4.9 46.7 1.0 O X:HOH2034 4.9 24.9 1.0

Magnesium binding site 5 out of 5 in the Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure Analysis of A Fluorescent Form of H-Ras P21 in Complex with Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg171 b:23.7 occ:0.50 O X:HOH2132 2.0 34.4 1.0 O X:HOH2041 2.0 44.9 0.5 O X:HOH2155 2.1 33.0 0.5 O X:HOH2156 2.6 48.0 1.0 O X:GLY138 3.9 24.1 1.0 O X:HOH2131 4.0 54.9 1.0 OE1 X:GLU162 4.2 30.9 1.0 OE2 X:GLU162 4.3 31.5 1.0 OE1 X:GLN165 4.5 36.8 1.0 CD X:GLU162 4.7 30.9 1.0 C X:GLY138 4.8 25.2 1.0

B.U.Klink, R.S.Goody, A.J.Scheidig. A Newly Designed Microspectrofluorometer For Kinetic Studies on Protein Crystals in Combination with X-Ray Diffraction Biophys.J. V. 91 981 2006.
ISSN: ISSN 0006-3495
PubMed: 16698776
DOI: 10.1529/BIOPHYSJ.105.078931