Magnesium in PDB 2hhu: C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position)

All present enzymatic activity of C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position):
2.7.7.7;

The structure of C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position), PDB code: 2hhu was solved by J.J.Warren, L.J.Forsberg, L.S.Beese, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	87.801, 93.415, 105.247, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 23.2

The binding sites of Magnesium atom in the C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position) (pdb code 2hhu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position), PDB code: 2hhu:

Magnesium binding site 1 out of 1 in the C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of C:O6-Methyl-Guanine in the Polymerase Postinsertion Site (-1 Basepair Position) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg920 b:29.2 occ:1.00 OD1 A:ASP653 2.7 18.3 1.0 O A:TYR654 2.9 13.1 1.0 O A:HOH2525 3.0 18.1 1.0 OD2 A:ASP830 3.1 16.0 1.0 O A:HOH3273 3.4 29.7 1.0 CG A:ASP653 3.6 19.2 1.0 O A:HOH3236 3.9 24.2 1.0 O A:HOH2512 3.9 16.2 1.0 CB A:ASP653 3.9 14.4 1.0 CG A:ASP830 4.0 16.6 1.0 O A:HOH2845 4.0 28.2 1.0 C A:TYR654 4.1 12.9 1.0 O A:HOH3089 4.2 28.5 1.0 OD1 A:ASP830 4.3 16.4 1.0 CA A:SER655 4.7 13.4 1.0 N A:TYR654 4.7 12.3 1.0 C A:ASP653 4.8 12.6 1.0 OD2 A:ASP653 4.8 19.4 1.0 N A:SER655 4.8 11.6 1.0 N A:GLN656 5.0 12.4 1.0 CA A:ASP653 5.0 12.4 1.0

J.J.Warren, L.J.Forsberg, L.S.Beese. The Structural Basis For the Mutagenicity of O6-Methyl-Guanine Lesions. Proc.Natl.Acad.Sci.Usa V. 103 19701 2006.
ISSN: ISSN 0027-8424
PubMed: 17179038
DOI: 10.1073/PNAS.0609580103