Magnesium in PDB 2j7q: Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate

The structure of Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate, PDB code: 2j7q was solved by C.Schlieker, W.A.Weihofen, E.Frijns, L.M.Kattenhorn, R.Gaudet, H.L.Ploegh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.8
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	40.907, 57.298, 67.279, 73.37, 85.37, 88.54
R / Rfree (%)	15.6 / 21.4

The binding sites of Magnesium atom in the Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate (pdb code 2j7q). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate, PDB code: 2j7q:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1234 b:8.8 occ:1.00 O A:HOH2047 2.3 16.4 1.0 O A:PRO41 2.3 9.5 1.0 O A:HOH2053 2.3 20.9 1.0 O A:HOH2045 2.4 28.7 1.0 OG A:SER47 2.4 11.6 1.0 OD2 A:ASP44 2.4 20.2 1.0 OD1 A:ASP44 2.7 15.8 1.0 CG A:ASP44 2.9 12.1 1.0 C A:PRO41 3.5 8.9 1.0 CB A:SER47 3.5 10.5 1.0 CA A:PRO41 4.1 9.4 1.0 O A:HOH2046 4.1 26.2 1.0 CB A:ASP44 4.4 9.6 1.0 N A:SER47 4.4 9.4 1.0 O A:HOH2052 4.4 44.9 1.0 CA A:SER47 4.5 9.9 1.0 CB A:PRO41 4.5 9.4 1.0 N A:VAL42 4.6 8.7 1.0 O A:HOH2054 4.7 30.1 1.0 CA A:VAL42 4.8 9.0 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1076 b:27.8 occ:1.00 O B:HOH2015 1.9 17.3 1.0 OE1 B:GLU18 2.2 20.2 1.0 O B:HOH2016 2.2 24.6 1.0 CD B:GLU18 3.1 16.6 1.0 OE2 B:GLU18 3.4 20.4 1.0 O B:HOH2019 3.8 20.3 1.0 CG B:GLU18 4.5 17.8 1.0 O B:HOH2018 4.6 29.4 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of the Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 in Complex with A Ubquitin-Based Suicide Substrate within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1076 b:31.6 occ:1.00 O D:HOH2027 2.1 23.3 1.0 O D:HOH2026 2.2 21.2 1.0 OE1 D:GLU18 2.2 22.2 1.0 CD D:GLU18 3.0 16.3 1.0 OE2 D:GLU18 3.2 21.2 1.0 CG D:GLU18 4.4 14.8 1.0 O D:HOH2029 4.5 25.1 1.0 CB D:GLU18 5.0 12.0 1.0

C.Schlieker, W.A.Weihofen, E.Frijns, L.M.Kattenhorn, R.Gaudet, H.L.Ploegh. Structure of A Herpesvirus-Encoded Cysteine Protease Reveals A Unique Class of Deubiquitinating Enzymes Mol.Cell V. 25 677 2007.
ISSN: ISSN 1097-2765
PubMed: 17349955
DOI: 10.1016/J.MOLCEL.2007.01.033