Magnesium in PDB 2zus: Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase

All present enzymatic activity of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase:
2.4.1.211;

The structure of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase, PDB code: 2zus was solved by M.Hidaka, M.Nishimoto, M.Kitaoka, T.Wakagi, H.Shoun, S.Fushinobu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.12 / 2.11
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	67.764, 111.483, 118.793, 105.13, 90.22, 107.78
R / Rfree (%)	17 / 22.5

The binding sites of Magnesium atom in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase (pdb code 2zus). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase, PDB code: 2zus:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg3001 b:32.7 occ:1.00 O B:HOH887 1.8 26.6 1.0 O B:HOH888 1.8 24.0 1.0 O B:HOH929 2.2 20.7 1.0 OD2 B:ASP134 3.7 34.8 1.0 OD2 B:ASP132 4.1 24.8 1.0 OD1 B:ASP132 4.1 25.1 1.0 CG B:ASP132 4.5 25.1 1.0 CG B:ASP134 4.6 30.7 1.0 CB B:ASP134 4.7 30.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg3002 b:35.1 occ:1.00 O D:HOH823 1.9 22.9 1.0 O D:HOH1081 1.9 33.5 1.0 O D:HOH959 2.0 30.5 1.0 O D:HOH931 2.1 22.7 1.0 OD2 D:ASP134 3.5 36.9 1.0 OD1 D:ASP132 3.9 22.8 1.0 OD2 D:ASP132 4.1 21.9 1.0 CG D:ASP132 4.4 23.2 1.0 CG D:ASP134 4.4 32.0 1.0 O D:HOH1708 4.4 34.5 1.0 CB D:ASP134 4.7 29.3 1.0

M.Hidaka, M.Nishimoto, M.Kitaoka, T.Wakagi, H.Shoun, S.Fushinobu. The Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase: A Large Deformation of A Tim Barrel Scaffold J.Biol.Chem. V. 284 7273 2009.
ISSN: ISSN 0021-9258
PubMed: 19124470
DOI: 10.1074/JBC.M808525200