Magnesium in PDB 3hjf: Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand

The structure of Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand, PDB code: 3hjf was solved by Y.Wang, H.Li, G.Sheng, D.J.Patel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.55 / 3.06
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	111.868, 111.868, 177.035, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 25.5

The binding sites of Magnesium atom in the Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand (pdb code 3hjf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand, PDB code: 3hjf:

Magnesium binding site 1 out of 1 in the Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of T. Thermophilus Argonaute E546 Mutant Protein Complexed with Dna Guide Strand and 15-Nt Rna Target Strand within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg22 b:65.8 occ:1.00 OXT A:VAL685 2.1 80.1 1.0 OP3 X:DT1 2.2 70.8 1.0 OP1 X:DA3 2.2 56.1 1.0 O A:VAL685 2.2 78.8 1.0 C A:VAL685 2.5 77.9 1.0 OP2 X:DT1 2.9 68.7 1.0 P X:DT1 3.0 60.5 1.0 P X:DA3 3.6 71.6 1.0 OP1 X:DT1 3.9 52.3 1.0 NZ A:LYS457 3.9 68.2 1.0 OE1 A:GLN433 4.0 71.6 1.0 CA A:VAL685 4.0 61.7 1.0 OP2 X:DA3 4.3 74.4 1.0 O5' X:DT1 4.3 64.3 1.0 O3' X:DG2 4.4 70.3 1.0 C5' X:DT1 4.5 63.6 1.0 O A:PHE684 4.6 86.8 1.0 O5' X:DA3 4.7 65.3 1.0 N A:VAL685 4.7 64.8 1.0 CD A:GLN433 4.8 67.8 1.0 CE A:LYS457 4.9 59.1 1.0 CB A:VAL685 4.9 67.5 1.0 NE2 A:GLN433 4.9 69.6 1.0 C A:PHE684 5.0 69.2 1.0

Y.Wang, S.Juranek, H.Li, G.Sheng, G.S.Wardle, T.Tuschl, D.J.Patel. Nucleation, Propagation and Cleavage of Target Rnas in Ago Silencing Complexes. Nature V. 461 754 2009.
ISSN: ISSN 0028-0836
PubMed: 19812667
DOI: 10.1038/NATURE08434