Magnesium in PDB 3klv: M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna

All present enzymatic activity of M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna:
2.7.7.48;

The structure of M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna, PDB code: 3klv was solved by C.Ferrer-Orta, M.Sierra, R.Agudo, R.Perez-Luque, A.Arias, N.Verdaguer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.60
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	93.657, 93.657, 99.428, 90.00, 90.00, 120.00
R / Rfree (%)	23.3 / 28.7

The binding sites of Magnesium atom in the M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna (pdb code 3klv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna, PDB code: 3klv:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg615 b:24.5 occ:1.00 OE1 A:GLU293 2.4 71.2 1.0 OD2 A:ASP63 2.9 64.2 1.0 CD A:GLU293 3.6 72.0 1.0 CG A:ASP63 3.8 63.9 1.0 CB A:GLU293 3.8 67.1 1.0 CB A:ASP63 4.1 65.8 1.0 CG A:GLU293 4.3 70.1 1.0 CD2 A:LEU2 4.4 72.1 1.0 OG A:SER250 4.4 56.7 1.0 OE2 A:GLU293 4.5 74.3 1.0 CD A:LYS65 4.7 64.5 1.0 O A:ASP63 4.8 65.9 1.0 OD1 A:ASP63 4.9 62.1 1.0

Magnesium binding site 2 out of 2 in the M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of M296I G62S Mutant of Foot-and-Mouth Disease Virus Rna-Polymerase in Complex with A Template- Primer Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1055 b:88.7 occ:1.00 OD2 A:ASP238 2.7 55.0 1.0 OD1 A:ASP339 2.8 50.2 1.0 O A:THR384 3.3 49.8 1.0 CG A:ASP238 3.5 54.7 1.0 OD1 A:ASP238 3.6 55.2 1.0 OD2 A:ASP240 3.6 52.7 1.0 CG A:ASP339 3.9 48.3 1.0 C A:THR384 4.3 49.7 1.0 O A:VAL239 4.4 51.0 1.0 OD2 A:ASP339 4.5 49.5 1.0 CG2 A:THR384 4.6 50.8 1.0 O A:ILE340 4.6 48.6 1.0 CG A:ASP240 4.8 54.3 1.0 CB A:THR384 4.8 51.9 1.0 N A:ILE340 4.8 46.9 1.0 CB A:ASP238 4.9 55.0 1.0 CB A:ALA367 4.9 64.8 1.0

C.Ferrer-Orta, M.Sierra, R.Agudo, I.De La Higuera, A.Arias, R.Perez-Luque, C.Escarmis, E.Domingo, N.Verdaguer. Structure of Foot-and-Mouth Disease Virus Mutant Polymerases with Reduced Sensitivity to Ribavirin J.Virol. V. 84 6188 2010.
ISSN: ISSN 0022-538X
PubMed: 20392853
DOI: 10.1128/JVI.02420-09