Atomistry » Magnesium » PDB 4an2-4ava » 4aq7
Atomistry »
  Magnesium »
    PDB 4an2-4ava »
      4aq7 »

Magnesium in PDB 4aq7: Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation

Enzymatic activity of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation

All present enzymatic activity of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation:
6.1.1.4;

Protein crystallography data

The structure of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation, PDB code: 4aq7 was solved by A.Palencia, T.Crepin, M.T.Vu, T.L.Lincecum Jr, S.A.Martinis, S.Cusack, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.69 / 2.50
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 158.660, 69.200, 228.840, 90.00, 104.35, 90.00
R / Rfree (%) 18.752 / 25.031

Other elements in 4aq7:

The structure of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation (pdb code 4aq7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation, PDB code: 4aq7:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 4aq7

Go back to Magnesium Binding Sites List in 4aq7
Magnesium binding site 1 out of 2 in the Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1077

b:55.8
occ:1.00
OP2 B:U8 2.0 33.5 1.0
OP2 B:G9 2.3 32.3 1.0
P B:U8 3.4 33.5 1.0
P B:G9 3.6 32.7 1.0
OP1 B:U11 4.0 36.6 1.0
O5' B:U8 4.1 33.9 1.0
OP1 B:U8 4.1 31.6 1.0
OP1 B:G9 4.3 34.2 1.0
C5' B:U8 4.3 32.5 1.0
O5' B:G9 4.5 32.7 1.0
OP2 B:G12 4.5 30.4 1.0
O3' B:A7 4.6 30.2 1.0
C5' B:G9 4.6 33.9 1.0
O3' B:U8 4.7 32.0 1.0
C3' B:U8 4.8 31.7 1.0

Magnesium binding site 2 out of 2 in 4aq7

Go back to Magnesium Binding Sites List in 4aq7
Magnesium binding site 2 out of 2 in the Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Ternary Complex of E. Coli Leucyl-Trna Synthetase, Trna(Leu) and Leucyl-Adenylate Analogue in the Aminoacylation Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Mg1077

b:67.7
occ:1.00
OP2 E:G9 2.2 52.5 1.0
OP2 E:U8 2.6 52.6 1.0
P E:G9 3.4 55.0 1.0
OP1 E:G9 3.8 56.9 1.0
P E:U8 3.9 50.5 1.0
OP1 E:U11 3.9 58.6 1.0
O5' E:U8 4.1 50.2 1.0
O5' E:G9 4.5 54.1 1.0
O3' E:U8 4.6 52.5 1.0
C5' E:G9 4.7 54.6 1.0
O3' E:A7 4.9 51.6 1.0
OP2 E:U11 4.9 59.3 1.0
OP1 E:U8 5.0 50.1 1.0
C5' E:U8 5.0 50.3 1.0
P E:U11 5.0 59.6 1.0

Reference:

A.Palencia, T.Crepin, M.T.Vu, T.L.Lincecum Jr, S.A.Martinis, S.Cusack. Structural Dynamics of the Aminoacylation and Proofreading Functional Cycle of Bacterial Leucyl-Trna Synthetase Nat.Struct.Mol.Biol. V. 19 677 2012.
ISSN: ISSN 1545-9993
PubMed: 22683997
DOI: 10.1038/NSMB.2317
Page generated: Thu Aug 15 14:39:42 2024

Last articles

Fe in 2YXO
Fe in 2YRS
Fe in 2YXC
Fe in 2YNM
Fe in 2YVJ
Fe in 2YP1
Fe in 2YU2
Fe in 2YU1
Fe in 2YQB
Fe in 2YOO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy