Magnesium in PDB 4bbr: Structure of Rna Polymerase II-Tfiib Complex

All present enzymatic activity of Structure of Rna Polymerase II-Tfiib Complex:
2.7.7.6;

The structure of Structure of Rna Polymerase II-Tfiib Complex, PDB code: 4bbr was solved by S.Sainsbury, J.Niesser, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.41 / 3.40
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	222.440, 386.760, 254.350, 90.00, 90.00, 90.00
R / Rfree (%)	17.5 / 21.1

The structure of Structure of Rna Polymerase II-Tfiib Complex also contains other interesting chemical elements:

Zinc

(Zn)

9 atoms

The binding sites of Magnesium atom in the Structure of Rna Polymerase II-Tfiib Complex (pdb code 4bbr). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Rna Polymerase II-Tfiib Complex, PDB code: 4bbr:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Rna Polymerase II-Tfiib Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Rna Polymerase II-Tfiib Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2458 b:75.9 occ:1.00 OD2 A:ASP481 2.1 0.8 1.0 OD2 A:ASP485 2.7 70.4 1.0 O A:ASN479 2.9 51.1 1.0 CG A:ASP481 3.1 89.7 1.0 N A:ASP481 3.3 57.0 1.0 C A:ALA480 3.6 56.8 1.0 CG A:ASP485 3.6 68.4 1.0 CB A:ASP481 3.6 62.7 1.0 CA A:ALA480 3.7 48.8 1.0 C A:ASN479 3.8 51.7 1.0 NH2 A:ARG446 3.8 81.3 1.0 OD1 A:ASP485 3.9 71.8 1.0 N A:ALA480 4.1 48.5 1.0 CA A:ASP481 4.1 58.6 1.0 CZ A:ARG446 4.1 93.5 1.0 NE A:ARG446 4.1 78.2 1.0 OD1 A:ASP481 4.2 92.8 1.0 O A:ALA480 4.4 56.3 1.0 O A:ASP481 4.5 62.2 1.0 OD1 A:ASP483 4.8 88.6 1.0 C A:ASP481 4.8 61.8 1.0 NH1 A:ARG446 4.9 79.2 1.0 CB A:ASP485 5.0 53.6 1.0

Magnesium binding site 2 out of 2 in the Structure of Rna Polymerase II-Tfiib Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Rna Polymerase II-Tfiib Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2459 b:80.7 occ:1.00 OD1 A:ASP481 2.2 92.8 1.0 CG A:ASP481 3.3 89.7 1.0 OE1 B:GLU836 3.7 0.8 1.0 OD2 A:ASP481 3.8 0.8 1.0 OE2 B:GLU836 4.1 0.1 1.0 O A:ALA480 4.3 56.3 1.0 CD B:GLU836 4.3 0.1 1.0 CB A:ASP481 4.5 62.7 1.0

S.Sainsbury, J.Niesser, P.Cramer. Structure and Function of the Initially Transcribing Rna Polymerase II-Tfiib Complex Nature V. 493 437 2013.
ISSN: ISSN 0028-0836
PubMed: 23151482
DOI: 10.1038/NATURE11715