Magnesium in PDB 4bwx: Structure of Neurospora Crassa PAN3 Pseudokinase Mutant

The structure of Structure of Neurospora Crassa PAN3 Pseudokinase Mutant, PDB code: 4bwx was solved by M.Christie, A.Boland, E.Huntzinger, O.Weichenrieder, E.Izaurralde, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.156 / 2.85
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	90.312, 90.312, 230.609, 90.00, 90.00, 120.00
R / Rfree (%)	21.1 / 24.24

The binding sites of Magnesium atom in the Structure of Neurospora Crassa PAN3 Pseudokinase Mutant (pdb code 4bwx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Neurospora Crassa PAN3 Pseudokinase Mutant, PDB code: 4bwx:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Neurospora Crassa PAN3 Pseudokinase Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Neurospora Crassa PAN3 Pseudokinase Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1648 b:0.2 occ:1.00 O3G A:AGS1647 2.4 0.6 1.0 O2A A:AGS1647 2.5 81.1 1.0 O2B A:AGS1647 2.7 0.4 1.0 OD1 A:ASP271 2.9 0.4 1.0 PB A:AGS1647 2.9 0.3 1.0 PG A:AGS1647 3.2 0.1 1.0 O A:SER285 3.3 88.9 1.0 CG A:ASP271 3.4 0.3 1.0 OD2 A:ASP271 3.4 98.3 1.0 O3B A:AGS1647 3.5 0.8 1.0 O3A A:AGS1647 3.9 0.5 1.0 C A:SER285 3.9 74.2 1.0 PA A:AGS1647 4.0 94.0 1.0 OG A:SER285 4.1 0.7 1.0 O5' A:AGS1647 4.1 77.3 1.0 C5' A:AGS1647 4.3 81.8 1.0 O1B A:AGS1647 4.3 93.6 1.0 O1A A:AGS1647 4.3 1.0 1.0 CA A:TRP286 4.4 61.1 1.0 N A:TRP286 4.4 59.7 1.0 CB A:SER285 4.4 88.1 1.0 N A:MET287 4.5 73.2 1.0 S1G A:AGS1647 4.5 86.1 1.0 O2G A:AGS1647 4.5 0.5 1.0 NH1 A:ARG302 4.5 99.0 1.0 C A:TRP286 4.6 70.1 1.0 CB A:ASP271 4.6 1.0 1.0 CB A:MET287 4.8 66.1 1.0 CA A:SER285 4.8 79.1 1.0 CG A:ARG276 4.9 0.9 1.0

Magnesium binding site 2 out of 2 in the Structure of Neurospora Crassa PAN3 Pseudokinase Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Neurospora Crassa PAN3 Pseudokinase Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1648 b:69.3 occ:1.00 O2G B:AGS1647 2.2 0.8 1.0 O2A B:AGS1647 2.3 72.9 1.0 PG B:AGS1647 2.8 1.0 1.0 OD2 B:ASP271 2.9 88.2 1.0 PB B:AGS1647 3.2 0.6 1.0 O3B B:AGS1647 3.4 0.9 1.0 O B:SER285 3.4 68.0 1.0 OG B:SER285 3.6 79.3 1.0 PA B:AGS1647 3.8 87.5 1.0 O1A B:AGS1647 3.9 98.4 1.0 NH1 B:ARG302 3.9 78.4 1.0 O2B B:AGS1647 3.9 0.4 1.0 O3G B:AGS1647 3.9 0.9 1.0 CG B:ASP271 4.0 88.6 1.0 O3A B:AGS1647 4.0 72.8 1.0 C B:SER285 4.0 62.6 1.0 O5' B:AGS1647 4.1 86.6 1.0 C5' B:AGS1647 4.4 81.2 1.0 OD1 B:ASP271 4.4 87.1 1.0 S1G B:AGS1647 4.4 0.9 1.0 CB B:SER285 4.4 62.8 1.0 NZ B:LYS413 4.5 71.2 1.0 CA B:TRP286 4.5 61.8 1.0 N B:TRP286 4.5 62.2 1.0 N B:MET287 4.5 70.8 1.0 C B:TRP286 4.6 75.0 1.0 CB B:MET287 4.7 58.4 1.0 O1B B:AGS1647 4.7 95.1 1.0 CA B:SER285 4.8 59.5 1.0 CG B:ARG276 4.9 0.7 1.0

M.Christie, A.Boland, E.Huntzinger, O.Weichenrieder, E.Izaurralde. Structure of the PAN3 Pseudokinase Reveals the Basis For Interactions with the PAN2 Deadenylase and the GW182 Proteins Mol.Cell V. 51 360 2013.
ISSN: ISSN 1097-2765
PubMed: 23932717
DOI: 10.1016/J.MOLCEL.2013.07.011