Magnesium in PDB 4mjy: Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q

All present enzymatic activity of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q:
1.16.3.1;

The structure of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q, PDB code: 4mjy was solved by S.Mangani, F.Di Pisa, C.Pozzi, P.Turano, D.Lalli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.06 / 1.40
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	183.790, 183.790, 183.790, 90.00, 90.00, 90.00
R / Rfree (%)	17.6 / 18.5

The structure of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q also contains other interesting chemical elements:

Iron	(Fe)	8 atoms
Chlorine	(Cl)	5 atoms

The binding sites of Magnesium atom in the Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q (pdb code 4mjy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q, PDB code: 4mjy:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg209 b:20.0 occ:0.80 O A:HOH424 2.0 18.3 0.8 O A:HOH547 2.1 24.6 0.8 O A:HOH344 2.1 12.4 0.8 O A:HOH482 2.1 23.6 0.8 O A:HOH492 2.1 22.0 0.8 OG A:SER10 2.2 12.4 1.0 CB A:SER10 3.3 11.1 1.0 CA A:SER10 3.9 10.5 1.0 O A:SER10 4.1 10.1 1.0 OE1 A:GLU13 4.1 9.5 1.0 O A:HOH370 4.3 17.5 1.0 O A:HOH589 4.3 33.6 1.0 C A:SER10 4.4 10.3 1.0 O A:HOH365 4.4 14.7 1.0 O A:HOH505 4.4 31.7 1.0 O A:HOH601 4.6 44.2 1.0 CD A:GLU13 4.6 8.9 1.0

Magnesium binding site 2 out of 3 in the Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg210 b:11.5 occ:0.40 O A:HOH625 1.7 12.2 0.4 O A:HOH554 1.8 17.1 0.4 O A:HOH576 2.1 27.9 0.4 O A:HOH624 2.3 19.4 0.4 O A:HOH352 2.4 5.2 0.4 O A:HOH595 2.7 24.4 0.4 O A:HOH437 4.0 21.5 1.0 O A:HOH484 4.0 33.0 1.0 OD1 A:ASN21 4.3 7.9 1.0 O A:HOH415 4.4 20.6 1.0 O A:HOH500 4.4 16.7 1.0 O A:ARG18 4.4 8.3 1.0 O A:HOH600 4.4 42.7 1.0 O A:HOH603 4.5 43.6 1.0 O A:HOH451 4.8 24.2 1.0 O A:HOH407 4.8 17.5 1.0 CB A:LEU22 4.9 8.3 1.0

Magnesium binding site 3 out of 3 in the Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Sixty Minutes Iron Loaded Frog M Ferritin Mutant H54Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg211 b:21.4 occ:0.60 O A:HOH635 2.1 24.6 0.6 O A:HOH555 2.1 21.6 0.6 O A:HOH597 2.1 25.8 0.6 O A:HOH488 2.1 28.2 1.0 O A:HOH637 2.3 25.3 0.6 O A:HOH636 2.3 35.7 0.6 O A:SER131 4.3 13.9 1.0 O A:HOH556 4.5 38.2 1.0 CA A:SER131 4.5 12.9 0.4 CA A:SER131 4.6 12.9 0.6 O A:GLU130 4.6 13.3 1.0 CB A:GLU135 4.7 14.2 1.0 C A:SER131 4.9 13.1 1.0 CG A:GLU135 4.9 15.4 1.0

S.Mangani, F.Di Pisa, C.Pozzi, P.Turano, D.Lalli. Crystallographic Snapshots of Iron in Frog M Ferritin To Be Published.