Magnesium in PDB 4tyn: Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef

All present enzymatic activity of Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef:
3.6.4.13;

The structure of Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef, PDB code: 4tyn was solved by A.L.Mallam, D.J.Sidote, A.M.Lambowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.15 / 2.96
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	90.389, 126.189, 55.231, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 24.4

The structure of Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef also contains other interesting chemical elements:

Fluorine

(F)

3 atoms

The binding sites of Magnesium atom in the Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef (pdb code 4tyn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef, PDB code: 4tyn:

Magnesium binding site 1 out of 1 in the Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dead-Box Helicase MSS116 Bound to Ssdna and Adp-Bef within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:32.7 occ:1.00 O3B A:ADP603 2.2 42.8 1.0 HZ1 A:LYS158 2.5 67.4 1.0 HA A:THR154 2.7 52.4 1.0 H A:GLY155 2.8 53.7 1.0 HH12 A:ARG469 2.8 57.0 1.0 HH22 A:ARG466 2.9 52.4 1.0 PB A:ADP603 3.1 35.2 1.0 HH22 A:ARG469 3.2 54.5 1.0 NZ A:LYS158 3.3 56.2 1.0 HG22 A:THR154 3.4 53.0 1.0 BE A:BEF602 3.4 34.2 1.0 HB A:THR154 3.5 61.7 1.0 HA2 A:GLY439 3.5 50.6 1.0 HZ3 A:LYS158 3.5 67.4 1.0 CA A:THR154 3.5 43.7 1.0 HE2 A:LYS158 3.5 55.6 1.0 O1B A:ADP603 3.6 35.6 1.0 N A:GLY155 3.6 44.8 1.0 F3 A:BEF602 3.6 36.1 1.0 NH1 A:ARG469 3.7 47.5 1.0 O2B A:ADP603 3.7 40.4 1.0 HH12 A:ARG466 3.7 51.0 1.0 NH2 A:ARG466 3.8 43.6 1.0 CB A:THR154 3.9 51.5 1.0 CE A:LYS158 3.9 46.3 1.0 HZ2 A:LYS158 3.9 67.4 1.0 NH2 A:ARG469 3.9 45.4 1.0 F1 A:BEF602 3.9 47.7 1.0 HE3 A:LYS158 4.0 55.6 1.0 H A:GLY439 4.0 46.2 1.0 C A:THR154 4.1 41.2 1.0 CG2 A:THR154 4.1 44.1 1.0 HB2 A:ALA306 4.2 61.7 1.0 OE2 A:GLU268 4.2 49.6 1.0 HH11 A:ARG469 4.3 57.0 1.0 CZ A:ARG469 4.3 43.5 1.0 HH21 A:ARG466 4.3 52.4 1.0 CA A:GLY439 4.4 42.1 1.0 NH1 A:ARG466 4.4 42.5 1.0 O3A A:ADP603 4.5 36.0 1.0 HA2 A:GLY155 4.5 55.4 1.0 CZ A:ARG466 4.6 41.4 1.0 N A:GLY439 4.6 38.5 1.0 N A:THR154 4.6 42.7 1.0 HH21 A:ARG469 4.6 54.5 1.0 HG21 A:THR154 4.7 53.0 1.0 CA A:GLY155 4.7 46.2 1.0 HG23 A:THR154 4.7 53.0 1.0 F2 A:BEF602 4.8 30.9 1.0 O A:LYS153 4.8 50.9 1.0 HB2 A:LYS158 4.9 47.4 1.0 HA3 A:GLY439 4.9 50.6 1.0 H A:THR156 5.0 45.8 1.0 O2A A:ADP603 5.0 41.5 1.0

A.L.Mallam, D.J.Sidote, A.M.Lambowitz. Molecular Insights Into Rna and Dna Helicase Evolution From the Determinants of Specificity For A Dead-Box Rna Helicase. Elife V. 4 2014.
ISSN: ESSN 2050-084X
PubMed: 25497230
DOI: 10.7554/ELIFE.04630