Magnesium in PDB 4tyy: Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef

All present enzymatic activity of Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef:
3.6.4.13;

The structure of Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef, PDB code: 4tyy was solved by A.L.Mallam, D.J.Sidote, A.M.Lambowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.07 / 2.74
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.635, 126.842, 55.303, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 26.7

The structure of Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef also contains other interesting chemical elements:

Fluorine

(F)

3 atoms

The binding sites of Magnesium atom in the Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef (pdb code 4tyy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef, PDB code: 4tyy:

Magnesium binding site 1 out of 1 in the Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dead-Box Helicase MSS116 Bound to Ssrna and Cdp-Bef within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:45.9 occ:1.00 F1 A:BEF601 1.9 85.1 1.0 O A:HOH724 2.1 66.4 1.0 O1B A:CDP602 2.2 61.6 1.0 O A:HOH725 2.3 56.1 1.0 O A:HOH726 2.3 57.5 1.0 HA2 A:GLY439 2.9 67.7 1.0 BE A:BEF601 3.3 84.9 1.0 PB A:CDP602 3.5 61.3 1.0 HE2 A:LYS158 3.6 76.9 1.0 OE2 A:GLU268 3.7 62.1 1.0 OD2 A:ASP267 3.8 51.1 1.0 CA A:GLY439 3.8 56.4 1.0 O3B A:CDP602 3.9 67.9 1.0 HB2 A:LYS158 3.9 70.6 1.0 OD1 A:ASP267 3.9 52.9 1.0 HA3 A:GLY439 4.0 67.7 1.0 HG1 A:THR159 4.0 64.1 1.0 O1A A:CDP602 4.2 55.7 1.0 O A:GLY439 4.2 58.6 1.0 HD11 A:LEU192 4.2 44.6 1.0 HH22 A:ARG469 4.3 77.0 1.0 F3 A:BEF601 4.3 86.7 1.0 F2 A:BEF601 4.3 48.1 1.0 OG1 A:THR159 4.3 53.4 1.0 CG A:ASP267 4.3 51.4 1.0 O2B A:CDP602 4.4 60.5 1.0 C A:GLY439 4.5 54.5 1.0 CE A:LYS158 4.6 64.1 1.0 O3A A:CDP602 4.6 69.0 1.0 H A:GLY439 4.6 60.2 1.0 HB3 A:LYS158 4.7 70.6 1.0 HD21 A:LEU192 4.7 43.7 1.0 CB A:LYS158 4.7 58.8 1.0 N A:GLY439 4.8 50.2 1.0 HE3 A:LYS158 4.8 76.9 1.0 HH21 A:ARG469 4.8 77.0 1.0 HZ3 A:LYS158 4.8 77.0 1.0 PA A:CDP602 4.8 57.3 1.0 CD A:GLU268 4.8 59.7 1.0 NH2 A:ARG469 4.9 64.2 1.0 H A:THR159 4.9 66.3 1.0 HD13 A:LEU192 4.9 44.6 1.0 HG2 A:GLU268 5.0 67.3 1.0

A.L.Mallam, D.J.Sidote, A.M.Lambowitz. Molecular Insights Into Rna and Dna Helicase Evolution From the Determinants of Specificity For A Dead-Box Rna Helicase. Elife V. 4 2014.
ISSN: ESSN 2050-084X
PubMed: 25497230
DOI: 10.7554/ELIFE.04630