Magnesium in PDB 4tz6: Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef

All present enzymatic activity of Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef:
3.6.4.13;

The structure of Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef, PDB code: 4tz6 was solved by A.L.Mallam, D.J.Sidote, A.M.Lambowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.21 / 3.21
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.757, 126.506, 55.510, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 27

The structure of Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef also contains other interesting chemical elements:

Fluorine

(F)

3 atoms

The binding sites of Magnesium atom in the Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef (pdb code 4tz6). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef, PDB code: 4tz6:

Magnesium binding site 1 out of 1 in the Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dead-Box Helicase MSS116 Bound to Ssrna and Udp-Bef within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:39.3 occ:1.00 F2 A:BEF601 2.2 81.1 1.0 O2B A:UDP602 2.4 61.9 1.0 OD2 A:ASP267 3.2 70.6 1.0 OD1 A:ASP267 3.2 54.8 1.0 OE2 A:GLU268 3.4 66.5 1.0 HA2 A:GLY439 3.5 67.2 1.0 BE A:BEF601 3.5 74.1 1.0 CG A:ASP267 3.6 67.5 1.0 PB A:UDP602 3.8 59.1 1.0 HD11 A:LEU192 4.1 52.7 1.0 OG1 A:THR159 4.1 49.4 1.0 O1B A:UDP602 4.2 85.3 1.0 F1 A:BEF601 4.3 79.5 1.0 HG2 A:GLU268 4.3 60.3 1.0 HA3 A:GLY439 4.3 67.2 1.0 CA A:GLY439 4.3 56.0 1.0 CD A:GLU268 4.4 63.6 1.0 HG1 A:THR159 4.4 59.3 1.0 HD21 A:LEU192 4.4 69.7 1.0 O1A A:UDP602 4.5 77.6 1.0 NZ A:LYS158 4.5 86.7 1.0 O3B A:UDP602 4.6 51.3 1.0 CB A:LYS158 4.6 57.2 1.0 F3 A:BEF601 4.6 59.0 1.0 H A:THR159 4.7 61.0 1.0 HE2 A:PHE162 4.8 69.3 1.0 O3A A:UDP602 4.8 67.1 1.0 O A:GLY439 4.8 54.5 1.0 CD A:LYS158 4.8 47.5 1.0 CE A:LYS158 4.9 79.4 1.0 CG A:GLU268 4.9 50.2 1.0 H A:GLU268 4.9 73.0 1.0 N A:THR159 5.0 50.8 1.0 CD1 A:LEU192 5.0 44.0 1.0

A.L.Mallam, D.J.Sidote, A.M.Lambowitz. Molecular Insights Into Rna and Dna Helicase Evolution From the Determinants of Specificity For A Dead-Box Rna Helicase. Elife V. 4 2014.
ISSN: ESSN 2050-084X
PubMed: 25497230
DOI: 10.7554/ELIFE.04630