Magnesium in PDB 5ahn: Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa

All present enzymatic activity of Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa:
1.1.1.205;

The structure of Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa, PDB code: 5ahn was solved by G.Labesse, T.Alexandre, M.Gelin, A.Haouz, H.Munier-Lehmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.857 / 1.65
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	116.551, 116.551, 58.247, 90.00, 90.00, 90.00
R / Rfree (%)	16.12 / 18.15

The binding sites of Magnesium atom in the Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa (pdb code 5ahn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa, PDB code: 5ahn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1469 b:30.0 occ:1.00 O A:HOH2102 1.9 38.3 1.0 O A:HOH2094 2.2 23.5 1.0 OE2 A:GLU54 2.2 21.6 1.0 O A:HOH2337 2.4 41.6 1.0 O A:HOH2336 2.5 29.0 1.0 O A:HOH2101 2.6 38.5 1.0 MG A:MG1470 2.7 30.0 1.0 O A:HOH2338 2.8 49.0 1.0 CD A:GLU54 3.2 25.1 1.0 OE1 A:GLU54 3.5 27.3 1.0 O A:HOH2335 3.6 45.2 1.0 NE2 A:HIS71 4.3 16.7 1.0 CG A:GLU54 4.6 17.1 1.0 CB A:PRO370 4.6 26.3 1.0 O A:HOH2103 4.6 48.5 1.0 O A:ASP50 4.7 22.9 1.0 O A:HOH2339 4.8 53.4 1.0 CE1 A:HIS71 4.9 15.0 1.0

Magnesium binding site 2 out of 2 in the Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Imp-Bound Form of the D199N Mutant of Impdh From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1470 b:30.0 occ:1.00 O A:HOH2101 2.0 38.5 1.0 O A:HOH2338 2.0 49.0 1.0 O A:HOH2337 2.0 41.6 1.0 O A:HOH2339 2.3 53.4 1.0 MG A:MG1469 2.7 30.0 1.0 O A:HOH2102 3.0 38.3 1.0 O A:HOH2094 3.9 23.5 1.0 O A:HOH2335 4.0 45.2 1.0 O A:HOH2290 4.2 48.2 1.0 O A:HOH2291 4.4 49.0 1.0 OE2 A:GLU54 4.5 21.6 1.0 O A:HOH2336 4.5 29.0 1.0 OE1 A:GLU54 4.6 27.3 1.0

G.Labesse, T.Alexandre, M.Gelin, A.Haouz, H.Munier-Lehmann. Crystallographic Studies of Two Variants of Pseudomonas Aeruginosa Impdh with Impaired Allosteric Regulation Acta Crystallogr.,Sect.D V. 71 1890 2015.
ISSN: ISSN 0907-4449
PubMed: 26327379
DOI: 10.1107/S1399004715013115