Magnesium in PDB 5bpm: Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K.

The structure of Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K., PDB code: 5bpm was solved by D.Narayanan, R.A.Engh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.08 / 1.83
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.466, 64.278, 144.860, 90.00, 90.00, 90.00
R / Rfree (%)	17.1 / 22.8

The structure of Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K. also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Sodium	(Na)	1 atom

The binding sites of Magnesium atom in the Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K. (pdb code 5bpm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K., PDB code: 5bpm:

Magnesium binding site 1 out of 1 in the Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Unhydrolyzed Atp Bound Human HSP70 Nbd Double Mutant E268Q+R272K. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:15.3 occ:1.00 O A:HOH591 2.3 12.5 1.0 O A:HOH609 2.4 11.7 1.0 O A:HOH621 2.4 11.3 1.0 O A:HOH649 2.4 17.2 1.0 OE2 A:GLU175 2.5 13.8 1.0 CD A:GLU175 3.1 14.2 1.0 HZ3 A:LYS71 3.3 15.3 1.0 HZ1 A:LYS71 3.5 15.3 1.0 OE1 A:GLU175 3.5 10.1 1.0 O A:HOH534 3.7 11.3 1.0 NZ A:LYS71 3.8 12.7 1.0 O3G A:ATP401 4.0 13.8 1.0 O A:HOH580 4.0 14.5 1.0 HG2 A:GLU175 4.0 12.6 1.0 OD2 A:ASP199 4.0 13.7 1.0 CG A:GLU175 4.1 10.5 1.0 HZ2 A:LYS71 4.1 15.3 1.0 HG3 A:PRO147 4.2 22.8 1.0 O A:HOH563 4.2 10.8 1.0 O3B A:ATP401 4.3 9.4 1.0 HB3 A:GLU175 4.3 9.4 1.0 O A:HOH552 4.3 15.7 1.0 O1B A:ATP401 4.4 14.1 1.0 HA2 A:GLY12 4.4 12.9 1.0 O A:HOH630 4.4 17.4 1.0 PG A:ATP401 4.6 12.9 1.0 OD1 A:ASP10 4.6 9.4 1.0 CB A:GLU175 4.6 7.9 1.0 HB2 A:GLU175 4.6 9.4 1.0 O1G A:ATP401 4.6 12.0 1.0 HG1 A:THR204 4.7 19.7 1.0 OD2 A:ASP206 4.7 16.6 1.0 HG21 A:VAL369 4.8 16.2 1.0 H A:THR13 4.9 15.6 1.0 CG A:ASP199 4.9 15.6 1.0 OG1 A:THR204 4.9 16.4 1.0 HG3 A:GLU175 4.9 12.6 1.0 PB A:ATP401 4.9 12.7 1.0

D.Narayanan, A.Pflug, T.Christopeit, P.Kyomuhendo, R.A.Engh. Nucleotide Binding to Variants of the HSP70-Nbd. To Be Published.