Magnesium in PDB 5hnk: Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions.

All present enzymatic activity of Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions.:
3.1.11.3;

The structure of Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions., PDB code: 5hnk was solved by F.A.Almalki, M.Feng, J.Zhang, S.E.Sedelnikova, J.B.Rafferty, J.R.Sayers, P.J.Artymiuk, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.19 / 2.22
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	44.720, 109.940, 127.340, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 23.4

The structure of Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions. also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Magnesium atom in the Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions. (pdb code 5hnk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions., PDB code: 5hnk:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions. within 5.0Å range: probe atom residue distance (Å) B Occ Y:Mg101 b:74.4 occ:1.00 O Y:HOH207 1.9 56.6 1.0 OP1 Y:DC6 2.0 56.1 1.0 O B:HOH423 2.1 77.5 1.0 O B:HOH450 2.2 47.8 1.0 O B:HOH409 2.2 48.3 1.0 P Y:DC6 3.4 43.6 1.0 O B:HOH413 3.6 74.3 1.0 OD1 B:ASP130 3.9 39.0 1.0 O B:HOH403 4.0 46.9 1.0 OD2 B:ASP68 4.0 53.3 1.0 OD1 B:ASP68 4.1 48.3 1.0 OP2 Y:DC6 4.1 43.5 1.0 O3' Y:DG5 4.2 45.1 1.0 O5' Y:DC6 4.4 41.4 1.0 OD2 B:ASP26 4.4 26.9 1.0 OD1 B:ASP26 4.4 31.1 1.0 CG B:ASP68 4.4 41.6 1.0 NZ B:LYS153 4.5 70.0 1.0 NZ B:LYS83 4.5 55.1 1.0 O B:HOH404 4.6 51.9 1.0 CD B:LYS153 4.6 59.0 1.0 C3' Y:DG5 4.8 39.0 1.0 CE B:LYS153 4.8 62.9 1.0 CG B:ASP26 4.9 29.5 1.0 CG B:ASP130 4.9 39.9 1.0 C4' Y:DG5 5.0 41.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of T5FEN in Complex Intact Substrate and Metal Ions. within 5.0Å range: probe atom residue distance (Å) B Occ Y:Mg102 b:47.3 occ:1.00 O Y:HOH205 2.1 45.1 1.0 OP2 Y:DG7 2.1 32.4 1.0 O Y:HOH218 2.1 53.5 1.0 O Y:HOH208 2.1 44.8 1.0 O Y:HOH212 2.3 43.2 1.0 O B:HOH462 2.3 49.4 1.0 P Y:DG7 3.5 30.8 1.0 OD1 B:ASP201 4.0 48.6 1.0 O3' Y:DC6 4.3 31.3 1.0 C3' Y:DC6 4.3 33.0 1.0 O Y:HOH222 4.3 45.5 1.0 OP1 Y:DG7 4.3 33.1 1.0 OD2 B:ASP201 4.3 64.3 1.0 C8 Y:DG7 4.4 31.1 1.0 N7 Y:DG7 4.5 29.0 1.0 O5' Y:DG7 4.5 34.3 1.0 CG B:ASP201 4.6 49.7 1.0 O Y:HOH203 4.8 37.5 1.0

F.A.Almalki, C.S.Flemming, J.Zhang, M.Feng, S.E.Sedelnikova, T.Ceska, J.B.Rafferty, J.R.Sayers, P.J.Artymiuk. Direct Observation of Dna Threading in Flap Endonuclease Complexes. Nat.Struct.Mol.Biol. V. 23 640 2016.
ISSN: ESSN 1545-9985
PubMed: 27273516
DOI: 10.1038/NSMB.3241