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Magnesium in PDB 5kqw: Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries

Enzymatic activity of Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries

All present enzymatic activity of Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries:
2.6.1.18;

Protein crystallography data

The structure of Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries, PDB code: 5kqw was solved by M.Wilding, J.Newman, T.S.Peat, C.Scott, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.20 / 2.23
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 66.051, 125.179, 110.496, 90.00, 101.97, 90.00
R / Rfree (%) 16.4 / 19.2

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries (pdb code 5kqw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries, PDB code: 5kqw:

Magnesium binding site 1 out of 1 in 5kqw

Go back to Magnesium Binding Sites List in 5kqw
Magnesium binding site 1 out of 1 in the Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins For New Chemistries within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg501

b:31.6
occ:1.00
 O A:HOH601 2.0 25.8 1.0
O A:HOH689 2.0 22.9 1.0
OD2 A:ASP218 2.4 26.7 1.0
CG A:ASP218 3.2 22.8 1.0
OD1 A:ASP218 3.2 23.2 1.0
OE1 A:GLN142 4.1 24.6 1.0
O A:HOH612 4.1 28.8 1.0
NE2 A:GLN142 4.2 26.8 1.0
CD A:GLN142 4.5 26.7 1.0
CB A:ASP218 4.7 18.6 1.0
CG2 A:THR219 4.8 16.1 1.0

Reference:

M.Wilding, T.S.Peat, S.Kalyaanamoorthy, J.Newman, C.Scott, L.S.Jermiin. Reverse Engineering: Transaminase Biocatalyst Development Using Ancestral Sequence Reconstruction Green Chemistry V. 19 5375 2017.
DOI: 10.1039/C7GC02343J
Page generated: Sun Sep 29 19:11:51 2024

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