Magnesium in PDB 6bt2: Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
Enzymatic activity of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
All present enzymatic activity of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion:
3.1.13.4;
Protein crystallography data
The structure of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion, PDB code: 6bt2
was solved by
E.T.Abshire,
J.Chasseur,
P.Del Rizzo,
R.Trievel,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
48.23 /
2.41
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
62.004,
69.437,
153.496,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
16.1 /
22.4
|
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
(pdb code 6bt2). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the
Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion, PDB code: 6bt2:
Jump to Magnesium binding site number:
1;
2;
3;
4;
Magnesium binding site 1 out
of 4 in 6bt2
Go back to
Magnesium Binding Sites List in 6bt2
Magnesium binding site 1 out
of 4 in the Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg501
b:37.4
occ:1.00
|
O
|
A:HOH641
|
1.7
|
26.5
|
1.0
|
O
|
A:HOH611
|
2.0
|
27.9
|
1.0
|
O
|
A:HOH644
|
2.0
|
19.8
|
1.0
|
O
|
A:HOH629
|
2.0
|
37.2
|
1.0
|
OE2
|
A:GLU195
|
2.3
|
34.1
|
1.0
|
O
|
A:HOH689
|
2.3
|
32.4
|
1.0
|
CD
|
A:GLU195
|
3.2
|
40.4
|
1.0
|
O
|
A:HOH701
|
3.4
|
20.6
|
1.0
|
OE1
|
A:GLU195
|
3.4
|
32.1
|
1.0
|
MG
|
A:MG502
|
3.8
|
19.8
|
1.0
|
O
|
A:HOH620
|
3.9
|
27.0
|
1.0
|
NE2
|
A:HIS414
|
4.0
|
30.4
|
1.0
|
ND2
|
A:ASN149
|
4.0
|
22.4
|
1.0
|
OD2
|
A:ASP413
|
4.0
|
47.9
|
1.0
|
O
|
A:HOH682
|
4.1
|
42.8
|
1.0
|
OD1
|
A:ASP413
|
4.2
|
37.9
|
1.0
|
OD1
|
A:ASN149
|
4.3
|
38.8
|
1.0
|
O
|
A:HOH661
|
4.4
|
44.8
|
1.0
|
CD2
|
A:HIS414
|
4.5
|
32.1
|
1.0
|
CD1
|
A:LEU151
|
4.5
|
27.6
|
1.0
|
CG
|
A:GLU195
|
4.5
|
37.9
|
1.0
|
CG
|
A:ASN149
|
4.6
|
32.2
|
1.0
|
CG
|
A:ASP413
|
4.6
|
45.4
|
1.0
|
O
|
A:HOH692
|
4.6
|
71.5
|
1.0
|
O
|
A:HOH696
|
4.8
|
33.9
|
1.0
|
O
|
A:HOH704
|
4.9
|
51.2
|
1.0
|
|
Magnesium binding site 2 out
of 4 in 6bt2
Go back to
Magnesium Binding Sites List in 6bt2
Magnesium binding site 2 out
of 4 in the Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg502
b:19.8
occ:1.00
|
O
|
A:HOH620
|
1.9
|
27.0
|
1.0
|
O
|
A:HOH641
|
2.1
|
26.5
|
1.0
|
O
|
A:HOH701
|
2.1
|
20.6
|
1.0
|
O
|
A:HOH692
|
2.7
|
71.5
|
1.0
|
OD1
|
A:ASN326
|
3.1
|
30.8
|
1.0
|
OD2
|
A:ASP324
|
3.1
|
36.8
|
1.0
|
NE2
|
A:HIS414
|
3.4
|
30.4
|
1.0
|
ND2
|
A:ASN326
|
3.5
|
30.0
|
1.0
|
CE1
|
A:HIS414
|
3.6
|
27.6
|
1.0
|
O
|
A:HOH704
|
3.7
|
51.2
|
1.0
|
CG
|
A:ASN326
|
3.7
|
29.6
|
1.0
|
MG
|
A:MG501
|
3.8
|
37.4
|
1.0
|
CG
|
A:ASP324
|
4.0
|
27.5
|
1.0
|
O
|
A:HOH689
|
4.1
|
32.4
|
1.0
|
OD1
|
A:ASP324
|
4.1
|
26.8
|
1.0
|
O
|
A:HOH696
|
4.2
|
33.9
|
1.0
|
O
|
A:HOH611
|
4.2
|
27.9
|
1.0
|
ND2
|
A:ASN149
|
4.2
|
22.4
|
1.0
|
NE2
|
A:HIS286
|
4.3
|
25.5
|
1.0
|
OE2
|
A:GLU195
|
4.5
|
34.1
|
1.0
|
CB
|
A:ALA289
|
4.5
|
25.6
|
1.0
|
O
|
A:HOH698
|
4.6
|
42.2
|
1.0
|
CD2
|
A:HIS414
|
4.7
|
32.1
|
1.0
|
CD1
|
A:LEU376
|
4.8
|
30.8
|
1.0
|
O
|
A:HOH644
|
4.8
|
19.8
|
1.0
|
ND1
|
A:HIS414
|
4.9
|
28.8
|
1.0
|
O
|
A:HOH625
|
4.9
|
29.0
|
1.0
|
|
Magnesium binding site 3 out
of 4 in 6bt2
Go back to
Magnesium Binding Sites List in 6bt2
Magnesium binding site 3 out
of 4 in the Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg501
b:34.6
occ:1.00
|
OE2
|
B:GLU195
|
2.0
|
26.7
|
1.0
|
O
|
B:HOH672
|
2.0
|
28.3
|
1.0
|
O
|
B:HOH639
|
2.0
|
25.1
|
1.0
|
O
|
B:HOH636
|
2.1
|
26.5
|
1.0
|
O
|
B:HOH708
|
2.2
|
31.1
|
1.0
|
O
|
B:HOH634
|
2.4
|
21.6
|
1.0
|
CD
|
B:GLU195
|
2.9
|
35.9
|
1.0
|
OE1
|
B:GLU195
|
3.2
|
25.9
|
1.0
|
O
|
B:HOH710
|
3.5
|
52.2
|
1.0
|
O
|
B:HOH673
|
3.6
|
38.0
|
1.0
|
MG
|
B:MG502
|
4.0
|
39.3
|
1.0
|
O
|
B:HOH631
|
4.1
|
30.9
|
1.0
|
ND2
|
B:ASN149
|
4.1
|
24.7
|
1.0
|
CG
|
B:GLU195
|
4.3
|
29.8
|
1.0
|
O
|
B:HOH706
|
4.3
|
35.5
|
1.0
|
OD1
|
B:ASN149
|
4.3
|
28.8
|
1.0
|
OD2
|
B:ASP413
|
4.4
|
42.9
|
1.0
|
O
|
B:HOH700
|
4.5
|
52.3
|
1.0
|
NE2
|
B:HIS414
|
4.5
|
41.4
|
1.0
|
OD1
|
B:ASP413
|
4.6
|
35.7
|
1.0
|
CG
|
B:ASN149
|
4.6
|
34.0
|
1.0
|
CD1
|
B:LEU151
|
4.9
|
29.4
|
1.0
|
CD1
|
B:LEU155
|
4.9
|
34.1
|
1.0
|
CG
|
B:ASP413
|
5.0
|
39.1
|
1.0
|
|
Magnesium binding site 4 out
of 4 in 6bt2
Go back to
Magnesium Binding Sites List in 6bt2
Magnesium binding site 4 out
of 4 in the Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Structure of the Human Nocturnin Catalytic Domain with Bound Sulfate Anion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg502
b:39.3
occ:1.00
|
O
|
B:HOH631
|
1.8
|
30.9
|
1.0
|
O
|
B:HOH710
|
1.9
|
52.2
|
1.0
|
O
|
B:HOH672
|
2.1
|
28.3
|
1.0
|
OD1
|
B:ASN326
|
2.7
|
27.7
|
1.0
|
OD2
|
B:ASP324
|
3.3
|
33.5
|
1.0
|
NE2
|
B:HIS414
|
3.6
|
41.4
|
1.0
|
CG
|
B:ASN326
|
3.7
|
31.2
|
1.0
|
CE1
|
B:HIS414
|
3.8
|
37.7
|
1.0
|
O
|
B:HOH708
|
3.8
|
31.1
|
1.0
|
ND2
|
B:ASN326
|
3.9
|
30.1
|
1.0
|
MG
|
B:MG501
|
4.0
|
34.6
|
1.0
|
O
|
B:HOH700
|
4.0
|
52.3
|
1.0
|
OD1
|
B:ASP324
|
4.1
|
38.8
|
1.0
|
CG
|
B:ASP324
|
4.1
|
36.8
|
1.0
|
O
|
B:HOH639
|
4.3
|
25.1
|
1.0
|
ND2
|
B:ASN149
|
4.3
|
24.7
|
1.0
|
NE2
|
B:HIS286
|
4.5
|
24.9
|
1.0
|
CD1
|
B:LEU376
|
4.5
|
39.4
|
1.0
|
OE2
|
B:GLU195
|
4.7
|
26.7
|
1.0
|
CD2
|
B:HIS414
|
5.0
|
33.8
|
1.0
|
|
Reference:
E.T Abshire,
J.Chasseur,
J.A.Bohn,
P.A.Del Rizzo,
P.L.Freddolino,
A.C.Goldstrohm,
R.C.Trievel.
The Structure of Human Nocturnin Reveals A Conserved Ribonuclease Domain That Represses Target Transcript Translation and Abundance in Cells. Nucleic Acids Res. V. 46 6257 2018.
ISSN: ESSN 1362-4962
PubMed: 29860338
DOI: 10.1093/NAR/GKY412
Page generated: Mon Sep 30 20:00:19 2024
|