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Magnesium in PDB 6dog: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C, PDB code: 6dog was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.27 / 1.29
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.574, 37.889, 62.247, 90.00, 96.22, 90.00
R / Rfree (%) 16.1 / 18.3

Other elements in 6dog:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C (pdb code 6dog). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C, PDB code: 6dog:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dog

Go back to Magnesium Binding Sites List in 6dog
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:18.3
occ:1.00
OD2 A:ASP71 2.0 16.7 1.0
OP3 b:C5 2.0 22.3 0.7
O A:HOH302 2.0 26.7 0.3
OD1 A:ASP192 2.0 22.0 1.0
O A:HOH310 2.1 21.3 1.0
O A:HOH323 2.1 20.3 1.0
OP1 b:C5 2.3 26.2 0.3
OP1 b:C5 2.4 20.4 0.7
P b:C5 2.7 19.4 0.7
CG A:ASP71 3.0 15.0 1.0
CG A:ASP192 3.2 21.9 1.0
HA A:ASP192 3.2 20.9 1.0
OD1 A:ASP71 3.4 14.4 1.0
P b:C5 3.5 18.6 0.3
HB2 A:ASP192 3.6 24.7 1.0
H A:TYR193 3.6 25.6 1.0
MG A:MG202 3.7 17.2 1.0
CB A:ASP192 3.7 20.6 1.0
OP2 b:C5 3.8 19.8 0.7
NZ A:LYS196 3.8 25.3 0.7
OP2 b:C5 3.9 20.6 0.3
CA A:ASP192 3.9 17.4 1.0
K A:K203 3.9 21.4 0.1
O5' b:C5 3.9 17.3 0.7
OP2 b:G6 4.0 21.9 1.0
O A:VAL72 4.0 16.6 1.0
O b:HOH102 4.1 28.9 0.9
OD2 A:ASP132 4.1 35.5 1.0
OE2 A:GLU188 4.2 42.5 1.0
HG22 A:ILE189 4.2 22.2 1.0
OD2 A:ASP192 4.2 23.4 1.0
C5' b:C5 4.3 20.4 1.0
O5' b:C5 4.3 24.5 0.3
CB A:ASP71 4.3 13.3 1.0
N A:TYR193 4.3 21.3 1.0
HB3 A:ASP71 4.4 15.9 1.0
O A:HOH316 4.5 21.5 0.3
HD1 A:TYR193 4.6 23.0 1.0
O3' B:U4 4.6 19.1 0.3
HB3 A:ASP192 4.7 24.7 1.0
HB2 A:ASP71 4.7 15.9 1.0
HG21 A:ILE189 4.7 22.2 1.0
C A:ASP192 4.7 22.3 1.0
CG2 A:ILE189 4.8 18.5 1.0
HG23 A:ILE189 4.9 22.2 1.0
K A:K217 4.9 22.8 0.3
HB2 A:TYR193 4.9 31.3 1.0
N A:ASP192 5.0 18.4 1.0

Magnesium binding site 2 out of 2 in 6dog

Go back to Magnesium Binding Sites List in 6dog
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 600 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:17.2
occ:1.00
OD1 A:ASP71 2.0 14.4 1.0
OE1 A:GLU109 2.0 15.4 1.0
OP1 b:C5 2.1 20.4 0.7
OP1 b:C5 2.1 26.2 0.3
OD1 A:ASP132 2.2 17.5 1.0
O3' B:U4 2.6 19.1 0.3
OD2 A:ASP132 2.7 35.5 1.0
CG A:ASP132 2.7 18.6 1.0
P b:C5 2.9 18.6 0.3
CD A:GLU109 3.1 13.4 1.0
CG A:ASP71 3.1 15.0 1.0
O A:HOH344 3.3 14.8 1.0
OE2 A:GLU109 3.3 14.1 1.0
P b:C5 3.4 19.4 0.7
OD2 A:ASP71 3.5 16.7 1.0
C3' B:U4 3.5 18.1 0.7
HG12 A:VAL72 3.5 20.3 1.0
O3' B:U4 3.6 19.5 0.7
MG A:MG201 3.7 18.3 1.0
O A:HOH323 3.7 20.3 1.0
H A:VAL72 3.8 17.9 1.0
O5' b:C5 3.9 24.5 0.3
OP2 b:C5 3.9 19.8 0.7
C3' B:U4 3.9 25.4 0.3
HA A:ASP71 4.0 15.6 1.0
C5' b:C5 4.1 20.4 1.0
O5' b:C5 4.1 17.3 0.7
OP2 b:C5 4.1 20.6 0.3
H A:ASP132 4.2 14.1 1.0
CB A:ASP132 4.2 14.8 1.0
HB2 A:SER133 4.3 16.1 1.0
O2' B:U4 4.3 17.5 0.3
OP3 b:C5 4.3 22.3 0.7
CB A:ASP71 4.3 13.3 1.0
HG21 A:ILE189 4.4 22.2 1.0
CG A:GLU109 4.4 12.7 1.0
C4' B:U4 4.4 24.8 0.3
C4' B:U4 4.5 24.9 0.7
CG1 A:VAL72 4.5 16.9 1.0
HG3 A:GLU109 4.5 15.2 1.0
N A:VAL72 4.5 14.9 1.0
HB2 A:ASP71 4.6 15.9 1.0
CA A:ASP71 4.6 13.0 1.0
HB3 A:ASP132 4.6 17.8 1.0
C2' B:U4 4.7 17.3 0.7
C5' B:U4 4.7 18.1 0.7
HB2 A:ASP132 4.7 17.8 1.0
C2' B:U4 4.7 18.6 0.3
HG2 A:GLU109 4.7 15.2 1.0
O A:HOH310 4.8 21.3 1.0
K A:K217 4.8 22.8 0.3
C5' B:U4 4.8 30.2 0.3
HG13 A:VAL72 4.8 20.3 1.0
O A:HOH302 4.8 26.7 0.3
HG11 A:VAL72 4.9 20.3 1.0
CA A:ASP132 4.9 13.4 1.0
N A:ASP132 4.9 11.8 1.0
O A:VAL72 4.9 16.6 1.0
HG22 A:ILE189 4.9 22.2 1.0
C A:ASP132 4.9 13.5 1.0
O2' B:U4 4.9 22.7 0.7

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Wed Aug 13 05:16:02 2025

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