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Magnesium in PDB 6dom: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C, PDB code: 6dom was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.94 / 1.43
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.618, 37.341, 62.242, 90.00, 96.36, 90.00
R / Rfree (%) 14.8 / 18.4

Other elements in 6dom:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 3 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C (pdb code 6dom). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C, PDB code: 6dom:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dom

Go back to Magnesium Binding Sites List in 6dom
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:22.3
occ:1.00
OD1 A:ASP71 2.1 21.0 1.0
OE1 A:GLU109 2.2 24.6 1.0
OP1 b:C5 2.2 30.3 0.4
OP1 b:C5 2.2 30.0 0.3
OD1 A:ASP132 2.3 22.2 1.0
O3' B:U4 2.6 23.1 0.4
O3' B:U4 2.8 24.7 0.2
OD2 A:ASP132 2.8 30.0 1.0
OP1 b:C5 2.9 28.8 0.2
CG A:ASP132 2.9 21.9 1.0
CD A:GLU109 3.1 22.2 1.0
P b:C5 3.1 26.2 0.4
CG A:ASP71 3.2 19.7 1.0
O A:HOH358 3.2 20.1 1.0
OE2 A:GLU109 3.3 21.8 1.0
P b:C5 3.4 21.0 0.2
C3' B:U4 3.4 32.9 0.3
O3' B:U4 3.5 19.8 0.3
HG12 A:VAL72 3.5 24.9 1.0
OD2 A:ASP71 3.6 23.5 1.0
P b:C5 3.6 47.0 0.3
O b:HOH302 3.7 29.5 1.0
H A:VAL72 3.8 24.2 1.0
MG b:MG201 3.8 23.4 1.0
C3' B:U4 3.9 24.1 0.4
C3' B:U4 4.0 21.2 0.2
C5' b:C5 4.0 23.6 1.0
O2' B:U4 4.0 19.6 0.2
O5' b:C5 4.1 20.8 0.4
O2' B:U4 4.1 21.0 0.4
O5' b:C5 4.2 21.0 0.2
OP2 b:C5 4.3 40.8 0.4
O5' b:C5 4.3 44.4 0.3
HB2 A:SER133 4.3 19.6 1.0
OP2 b:C5 4.3 45.9 0.3
C4' B:U4 4.3 19.4 0.2
C4' B:U4 4.3 20.5 0.4
CB A:ASP132 4.4 19.5 1.0
CB A:ASP71 4.4 18.4 1.0
C4' B:U4 4.4 21.4 0.3
HG21 A:ILE189 4.4 26.1 1.0
CG1 A:VAL72 4.5 20.8 1.0
CG A:GLU109 4.5 17.9 1.0
C2' B:U4 4.5 22.3 0.3
N A:VAL72 4.6 20.2 1.0
C5' B:U4 4.6 34.5 0.3
OP2 b:C5 4.6 22.1 0.2
C2' B:U4 4.6 20.6 0.4
OP3 b:C5 4.6 37.1 0.3
C2' B:U4 4.6 18.9 0.2
HG3 A:GLU109 4.7 21.5 1.0
CA A:ASP71 4.7 17.4 1.0
HG2 A:GLU109 4.8 21.5 1.0
HG13 A:VAL72 4.8 24.9 1.0
HG11 A:VAL72 4.8 24.9 1.0
O2' B:U4 4.8 34.9 0.3
K A:K203 4.8 26.9 0.2
O b:HOH301 4.9 25.6 1.0
HG22 A:ILE189 4.9 26.1 1.0
N A:ASP132 4.9 15.6 1.0
O A:VAL72 5.0 21.4 1.0
C5' B:U4 5.0 20.6 0.4
C5' B:U4 5.0 20.6 0.2
CA A:ASP132 5.0 18.9 1.0

Magnesium binding site 2 out of 2 in 6dom

Go back to Magnesium Binding Sites List in 6dom
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 50 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
b:Mg201

b:23.4
occ:1.00
OP1 b:C5 1.9 28.8 0.2
OD1 A:ASP192 1.9 28.4 1.0
OP3 b:C5 2.0 37.1 0.3
O A:HOH306 2.0 28.6 0.7
O b:HOH301 2.1 25.6 1.0
O b:HOH302 2.1 29.5 1.0
OD2 A:ASP71 2.1 23.5 1.0
OP1 b:C5 2.2 30.3 0.4
OP1 b:C5 2.2 30.0 0.3
P b:C5 2.5 47.0 0.3
CG A:ASP71 3.1 19.7 1.0
CG A:ASP192 3.1 26.6 1.0
P b:C5 3.2 26.2 0.4
P b:C5 3.3 21.0 0.2
HA A:ASP192 3.3 30.0 1.0
OD1 A:ASP71 3.4 21.0 1.0
OP2 b:C5 3.5 45.9 0.3
H A:TYR193 3.5 31.1 1.0
OP2 b:C5 3.6 40.8 0.4
K A:K202 3.6 29.2 0.2
HB2 A:ASP192 3.8 31.1 1.0
CB A:ASP192 3.8 25.9 1.0
MG A:MG201 3.8 22.3 1.0
O5' b:C5 3.8 44.4 0.3
OP2 b:G6 4.0 28.5 1.0
OP2 b:C5 4.0 22.1 0.2
CA A:ASP192 4.0 25.0 1.0
O A:HOH345 4.0 30.9 0.4
OD2 A:ASP192 4.1 29.9 1.0
O5' b:C5 4.1 21.0 0.2
O5' b:C5 4.1 20.8 0.4
O A:VAL72 4.1 21.4 1.0
HG22 A:ILE189 4.2 26.1 1.0
OE2 A:GLU188 4.2 62.1 1.0
N A:TYR193 4.3 25.9 1.0
OD2 A:ASP132 4.3 30.0 1.0
C5' b:C5 4.4 23.6 1.0
O3' B:U4 4.4 24.7 0.2
CB A:ASP71 4.4 18.4 1.0
O3' B:U4 4.4 23.1 0.4
HD1 A:TYR193 4.6 30.6 1.0
K A:K203 4.7 26.9 0.2
C A:ASP192 4.7 26.2 1.0
HG21 A:ILE189 4.7 26.1 1.0
O A:HOH323 4.7 31.4 0.6
HB3 A:ASP192 4.7 31.1 1.0
O b:HOH303 4.8 29.0 0.6
HB2 A:TYR193 4.8 40.6 1.0
CG2 A:ILE189 4.8 21.7 1.0
H A:GLY194 4.9 40.8 1.0
HG23 A:ILE189 4.9 26.1 1.0
CE A:LYS196 5.0 43.0 0.7

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:19:58 2024

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