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Magnesium in PDB 6dop: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1)

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1)

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1):
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1), PDB code: 6dop was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.87 / 1.25
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.535, 37.625, 61.837, 90.00, 95.84, 90.00
R / Rfree (%) 16.2 / 17.8

Other elements in 6dop:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1) also contains other interesting chemical elements:

Potassium (K) 2 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1) (pdb code 6dop). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1), PDB code: 6dop:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dop

Go back to Magnesium Binding Sites List in 6dop
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:24.4
occ:1.00
OD1 A:ASP71 2.1 23.7 1.0
OP1 b:C5 2.2 27.6 0.3
OP1 b:C5 2.2 22.8 0.5
OE1 A:GLU109 2.4 27.7 1.0
OD1 A:ASP132 2.4 20.8 1.0
O3' B:U4 2.6 19.6 0.5
OP1 b:C5 2.7 35.2 0.2
O3' B:U4 2.7 19.8 0.2
OD2 A:ASP132 3.0 24.8 1.0
CG A:ASP132 3.1 21.8 1.0
P b:C5 3.1 23.6 0.5
CG A:ASP71 3.1 22.7 1.0
CD A:GLU109 3.2 22.5 1.0
P b:C5 3.3 20.8 0.2
O A:HOH350 3.3 18.8 1.0
OE2 A:GLU109 3.4 20.8 1.0
O A:HOH313 3.5 30.4 1.0
C3' B:U4 3.5 40.3 0.3
HG12 A:VAL72 3.5 24.9 1.0
OD2 A:ASP71 3.5 26.6 1.0
P b:C5 3.6 71.8 0.3
H A:VAL72 3.6 22.8 1.0
O3' B:U4 3.7 33.6 0.3
MG A:MG214 3.8 26.6 1.0
C3' B:U4 3.9 19.4 0.5
C3' B:U4 4.0 19.4 0.2
C5' b:C5 4.1 21.9 1.0
O5' b:C5 4.1 20.9 0.5
O2' B:U4 4.1 19.3 0.2
O2' B:U4 4.1 18.5 0.5
O5' b:C5 4.2 20.9 0.2
OP2 b:C5 4.2 21.4 0.3
OP2 b:C5 4.2 40.1 0.5
O5' b:C5 4.3 54.3 0.3
C4' B:U4 4.3 19.9 0.5
C4' B:U4 4.3 19.4 0.2
CB A:ASP71 4.3 18.6 1.0
C4' B:U4 4.4 26.0 0.3
HB2 A:SER133 4.4 19.5 1.0
CG1 A:VAL72 4.4 20.8 1.0
HG21 A:ILE189 4.5 28.4 1.0
N A:VAL72 4.5 19.0 1.0
OP2 b:C5 4.5 21.7 0.2
OP3 b:C5 4.5 39.6 0.3
CB A:ASP132 4.5 17.5 1.0
C5' B:U4 4.5 30.8 0.3
C2' B:U4 4.5 23.5 0.3
O A:HOH310 4.6 28.6 1.0
C2' B:U4 4.6 19.0 0.5
C2' B:U4 4.7 19.0 0.2
CA A:ASP71 4.7 16.5 1.0
CG A:GLU109 4.7 18.6 1.0
HG13 A:VAL72 4.7 24.9 1.0
HG11 A:VAL72 4.8 24.9 1.0
O2' B:U4 4.8 40.3 0.3
HG3 A:GLU109 4.9 22.3 1.0
C5' B:U4 4.9 20.0 0.5
O A:VAL72 4.9 19.9 1.0
C5' B:U4 4.9 20.0 0.2
HG2 A:GLU109 4.9 22.3 1.0

Magnesium binding site 2 out of 2 in 6dop

Go back to Magnesium Binding Sites List in 6dop
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (Dataset 1) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg214

b:26.6
occ:1.00
O A:HOH310 2.0 28.6 1.0
OD1 A:ASP192 2.0 31.1 1.0
OP1 b:C5 2.0 35.2 0.2
O A:HOH304 2.0 28.8 0.7
OD2 A:ASP71 2.1 26.6 1.0
OP3 b:C5 2.1 39.6 0.3
OP1 b:C5 2.2 22.8 0.5
O A:HOH313 2.2 30.4 1.0
OP1 b:C5 2.4 27.6 0.3
P b:C5 2.8 71.8 0.3
CG A:ASP71 3.1 22.7 1.0
CG A:ASP192 3.2 30.6 1.0
P b:C5 3.3 23.6 0.5
HA A:ASP192 3.3 31.2 1.0
P b:C5 3.3 20.8 0.2
OD1 A:ASP71 3.5 23.7 1.0
K A:K202 3.5 44.5 0.2
OP2 b:C5 3.6 40.1 0.5
H A:TYR193 3.6 38.0 1.0
MG A:MG201 3.8 24.4 1.0
OP2 b:C5 3.8 21.4 0.3
HB2 A:ASP192 3.9 35.2 1.0
OP2 b:C5 3.9 21.7 0.2
CB A:ASP192 3.9 29.3 1.0
O5' b:C5 4.0 54.3 0.3
OP2 b:G6 4.0 32.7 1.0
O A:HOH319 4.0 35.1 0.4
CA A:ASP192 4.0 26.0 1.0
O A:VAL72 4.1 19.9 1.0
OD2 A:ASP192 4.1 37.2 1.0
OE2 A:GLU188 4.2 81.9 1.0
O5' b:C5 4.2 20.9 0.5
O A:HOH308 4.2 37.1 0.6
O5' b:C5 4.2 20.9 0.2
HG22 A:ILE189 4.3 28.4 1.0
N A:TYR193 4.4 31.7 1.0
O3' B:U4 4.4 19.8 0.2
CB A:ASP71 4.4 18.6 1.0
O3' B:U4 4.4 19.6 0.5
OD2 A:ASP132 4.5 24.8 1.0
O b:HOH102 4.5 31.5 0.6
C5' b:C5 4.6 21.9 1.0
HD1 A:TYR193 4.7 31.5 1.0
HG21 A:ILE189 4.7 28.4 1.0
OE1 A:GLU188 4.7 76.8 1.0
C A:ASP192 4.8 26.5 1.0
CD A:GLU188 4.8 77.3 1.0
HB3 A:ASP192 4.8 35.2 1.0
HB2 A:TYR193 4.8 38.6 1.0
CG2 A:ILE189 4.9 23.7 1.0
H A:VAL72 4.9 22.8 1.0
H A:GLY194 4.9 45.6 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Wed Aug 13 05:16:57 2025

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